Analiza crijevne mikrobiote skupine ljudi s područja grada Zagreba te usporedba s crijevnim mikrobiotama okarakteriziranih svjetskih populacija

master's thesis

• Title (english): Analysis of the gut microbiota of a group of people from the city of Zagreb and comparison with the gut microbiota of characterized world populations
• Author: Marta Salopek
• Mentor: Antonio Starčević (mentor)
• Committee member: Blaženka Kos (predsjednik povjerenstva)
• Committee member: Jurica Žučko (član povjerenstva)
• Committee member: Igor Slivac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2023-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Crijevna mikrobiota složena je zajednica mikroorganizama koja ima važnu ulogu u probavi, sintezi vitamina, obrani od patogena i održavanju epitelne barijere. Čimbenici koji utječu na njen sastav, a time i na funkcije, mnogobrojni su. Napredci u metodama sekvenciranja i analizama podataka omogućuju njihovo dublje razumijevanje, kao i usporedbu sastava mikrobiote među različitim populacijama. Ova su istraživanja od neizmjerne važnosti jer pružaju temelje za očuvanje zdravlja i prilagođene medicinske intervencije. U ovom je radu provedena usporedba sastava mikrobiote populacije u gradu Zagrebu s drugim populacijskim skupinama upotrebom 16S rRNA sekvenciranja uz Illumina MiSeq te bioinformatičku platformu QIIME 2. U skladu s literaturom, utvrđeno je da su dva najzastupljenija koljena Firmicutes i Bacteroidetes, dok je najzastupljeniji rod Bacteroides. Sastav crijevne mikrobiote promatrane populacije podudara se sa sastavom drugih populacija koje također žive u urbanim područjima. Opsežnija su istraživanja svakako nužna, kao i usklađivanje metodoloških pristupa na globalnoj razini.

Abstract (english)

Gut microbiota is a complex community of microorganisms crucial for digestion, vitamin synthesis, defense against pathogens and maintaining the epithelial barrier. Various factors influence its composition and functions. Advances in sequencing techniques and data analysis have enabled a profound understanding and cross-population comparisons of microbiota composition. These studies are crucial for preserving health and tailoring medical interventions. In this research, a comparison of the microbiota composition of the population in the city of Zagreb with other population groups was carried out using 16S rRNA sequencing with Illumina MiSeq and the bioinformatics platform QIIME 2. As expected, Firmicutes and Bacteroidetes are dominant, with Bacteroides as the most abundant genus. The intestinal microbiota composition of this population aligns with other urban populations. Further comprehensive research is undoubtedly essential, along with the global coordination of methodological approaches.

• Keywords: crijevna mikrobiota; 16S rRNA sekvenciranje; QIIME 2; mikrobiom
• Keywords (english): gut microbiota; 16S rRNA sequencing; QIIME 2; microbiome
• Language: croatian
• URN:NBN: urn:nbn:hr:159:373316
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-07 07:30:48