Proizvodnja 2,3-butandiola u bioreaktoru s čvrstim slojem u uvjetima povećane inicijalne koncentracije octene kiseline

master's thesis

• Title (english): Production of 2,3-butanediol in a fixed-bed bioreactor under conditions of increased initial acetic acid concentration
• Author: Anita Galović
• Mentor: Mladen Pavlečić (mentor)
• Committee member: Antonija Trontel (predsjednik povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Committee member: Blaženka Kos (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2023-12-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

2,3-butandiol (2,3-BTD) je organska molekula koja se zbog svojih svojstava može koristiti kao gorivo ili aditiv gorivu, a nema štetnih učinaka na okoliš poput fosilnih goriva. Može se proizvesti kemijskom sintezom ili uz pomoć određenih vrsta mikroorganizama. U ovom radu proučavana je mogućnost biotehnološke proizvodnje 2,3-BTD-a pomoću bakterije Paenibacillus polymyxa DSM 742 na kemijski definiranim hranjivim podlogama uz povećanu inicijalnu koncentraciju octene kiseline (10 g/L, 12 g/L i 15 g/L) te je praćen njen utjecaj na prinos 2,3-BTD-a. Nakon preliminarnog dijela istraživanja u tikvicama, u drugom dijelu izrade ovog diplomskog rada, proučavana je mogućnost proizvodnje 2,3- BTD-a uz pomoć navedenog mikroorganizma u bioreaktoru s čvrstim slojem pri čemu su kao nosač/sredstvo za imobilizaciju stanica korišteni aktivni ugljen te bukove strugotine. U tikvicama su ostvareni prinosi redom iznosili 0,4888 g/L, 0,2433 g/L i 0,1617 g/L što pokazuje negativan utjecaj povećanja koncentracije octene kiseline na prinos 2,3-BTD-a u ovom slučaju. Što se tiče eksperimenata u bioreaktoru s čvrstim slojem, najveći prinos postignut je na bukovim strugotinama gdje je inicijalna koncentracija octene kiseline iznosila 15 g/L i on je iznosio 2,9368 g/L. Najmanji prinos, s druge strane, dobiven je u eksperimentu s 10 g/L octene kiseline na aktivnom ugljenu te je iznosio 0,6154 g/L. Međutim, u odnosu na rezultate ostvarene u preliminarnom istraživanju, ovdje nije uočen negativan utjecaj povećane inicijalne koncentracije octene kiseline i maksimalne koncentracije 2,3-BTD-a ostvarene u eksperimentima. Na temelju rezultata ovog diplomskog rada može se zaključiti da se oba imobilizacijska sredstva mogu koristiti u biotehnološkoj proizvodnji 2,3-BTD-a te da su se, u smislu ostvarivanja boljih pokazatelja uspješnosti, bukove strugotine pokazale kao bolje imobilizacijsko sredstvo.

Abstract (english)

2,3-butanediol (2,3-BTD) is an organic molecule that, due to its properties, can be used as a fuel or a fuel additive, and has no harmful effects on the environment like fossil fuels. It can be produced by chemical synthesis or by using different types of microorganism. In this paper, the possibility of biotechnological production of 2,3-BTD using the bacterium Paenibacillus polymyxa DSM 742 on chemically defined nutrient media with an increased initial concentration of acetic acid (10 g/L, 12 g/L and 15 g/L) and its influence on the yield of 2,3-BTD was studied. After preliminary part of the research in the flasks, in the second part of this thesis, the possibility of producing 2,3-BTD with the mentioned microorganism in a fixed-bed bioreactor was studied, where activated carbon and beech shavings were used as a carrier for cell immobilization. In the flasks, the yields were 0.4888 g/L, 0.2433 g/L and 0.1617 g/L, which shows the negative impact of increasing concentration of acetic acid on the yield of 2,3-BTD. Regarding the experiments in the fixed bed bioreactor, the highest yield was achieved on beech shavings where the initial concentration of acetic acid was 15 g/L and it was 2.9368 g/L. The lowest yield, on the other hand, was obtained in the experiment with 10 g/L acetic acid on activated carbon and was 0.6154 g/L. However, unlike the results achieved in the preliminary research, no negative influence of the increased initial concentration of acetic acid on the maximum concentration of 2,3-BTD achieved in the experiments was observed. Based on the results of this thesis, it can be concluded that both immobilization agents can be used in the biotechnological production of 2,3-BTD and that, in terms of achieving better performance indicators, beech shavings proved to be a better immobilization agent.

• Keywords: 23-butandiol; Paenibacillus polymyxa DSM 742; octena kiselina; bioreaktor s čvrstim slojem
• Keywords (english): 23-butanediol; Paenibacillus polymyxa DSM 742; acetic acid; fixed-bed bioreactor
• Language: croatian
• URN:NBN: urn:nbn:hr:159:586446
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-18 09:38:03