Utjecaj izvora ugljika na rast i proizvodnju šećernih alkohola tijekom uzgoja odabranih sojeva Kluyveromyces kvasaca

master's thesis

• Title (english): The influence of different carbon sources on the growth and sugar alcohol production during cultivation of selected Kluyveromyces strains
• Author: Mija Kaselj
• Mentor: Mario Novak (mentor)
• Mentor: Antonija Trontel (pomoć pri izradi, neposredni voditelj)
• Mentor: Nenad Marđetko (pomoć pri izradi, neposredni voditelj)
• Committee member: Antonija Trontel (predsjednik povjerenstva)
• Committee member: Bojan Žunar (član povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2023-06-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu ispitivan je utjecaj izvora ugljika na rast i sintezu proizvoda metabolizma tri odabrana soja kvasca Kluyveromyces marxianus: NBRC 1777, DKMU 3-1042 te CBS 712. Rastom na glukozi, najveći prinos etanola (1,01 g/L) ostvario je kvasac soja DKMU 3-1042, dok je kvasac soja NBRC 1777 ostvario najveći prinos arabitola (2,84 g/L) rastom na arabinozi, ksilitola (2,89 g/L) rastom na ksilozi, te etanola (1,15 g/L) rastom na glicerolu. Šaržnim uzgojem kvasca soja NBRC 1777, u bioreaktoru s miješalom, na simulatu hidrolizata izluženih rezanca šećerne repe proizvedeno je 5,13 g/L etanola u aerobnim uvjetima, dok je u anaerobnim proizvedeno 5,31 g/L etanola. Šaržnim uzgojem kvasca soja NBRC 1777, u bioreaktoru s miješalom, na kiselinskom hidrolizatu izluženih rezanca šećerne repe proizvedeno je 1,43 g/L etanola i 0,82 g/L octene kiseline u aerobnim uvjetima, dok je u anaerobnim uvjetima proizvedeno 1,28 g/L etanola te 4,46 g/L octene kiseline.

Abstract (english)

In this study, the influence of selected carbon sources in different concentrations on the growth and synthesis of metabolic products of three selected strains of Kluyveromyces marxianus: NBRC 1777, DKMU 3-1042 and CBS 712 was investigated. When grownon glucose, the highest yield of ethanol (1.01 g/L) was observed by yeast strain DKMU 3-1042, while yeast strain NBRC 1777 achieved the highest yield of arabitol (2.84 g/L) when grown on arabinose, xylitol (2, 89 g/L) when grown on xylose, and ethanol (1.15 g/L) when grown on glycerol. Batch cultivation of strain NBRC 1777 in a stirred tank bioreactor (STBR), on a simulated acid hydrolysate of leached sugar beet pulp, produced 5.13 g/L of ethanol under aerobic conditions, while 5.31 g/L of ethanol were produced under anaerobic conditions. Batch cultivation of strain NBRC 1777 in a STBR on acid hydrolysate of leached sugar beet pulp produced 1.43 g/L of ethanol under aerobic conditions, while in anaerobic conditions 1.28 g/L of ethanol were produced.

• Keywords: Kluyveromyces marxianus; izluženi rezanci šećerne repe; glicerol; šećerni alkoholi; bioreaktor s miješalom
• Keywords (english): Kluyveromyces marxianus; sugar beet pulp; glycerol; sugar alcohols; stirred tank bioreactor
• Language: croatian
• URN:NBN: urn:nbn:hr:159:132733
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-20 13:43:39