In vitro učinci resveratrola i ferocenskog derivata resveratrola na kulture stanica ovarija i keratinocita

master's thesis

• Title (english): In vitro effects of resveratrol and ferrocene derivative of resveratrol on ovarian and keratinocyte cell cultures
• Author: Bruno Doskočil
• Mentor: Ivana Kmetič (mentor)
• Mentor: Marina Miletić (pomoć pri izradi, neposredni voditelj)
• Mentor: Teuta Murati (pomoć pri izradi, neposredni voditelj)
• Committee member: Teuta Murati (predsjednik povjerenstva)
• Committee member: Kristina Radošević (član povjerenstva)
• Committee member: Igor Slivac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2023-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu ispitan je učinak resveratrola (RSV) te ferocenskog derivata resveratrola (RF) (5, 20, 50 i 100 µM) na indukciju stanične smrti u kulturama stanica ovarija (CHO-K1 stanična linija) i keratinocita (HaCaT stanična linija). Uz to, praćena je i vijabilnost te proliferacija stanica metodama Neutral Red i Trypan Blue. Nakon tretmana stanica RSV-om u obje stanične linije zabilježene su niže IC vrijednosti dobivene Trypan Blue metodom u odnosu na Neutral Red test što se može objasniti povećanjem broja i veličine lizosoma te većom količinom vezane boje Neutral Red u tretiranih stanica u odnosu na netretirane. Tretman HaCaT stanica RSV-om rezultirao je blago povećanim udjelom stanica u apoptozi u odnosu na kontrolni uzorak, dok je u CHO-K1 kulturi stanica taj učinak bio izraženiji i statistički značajan (u dozama ≥20 μM ). RSV je potaknuo autofagiju u obje kulture stanica, a vrijednost autophagy induction ratio varira ovisno o primijenjenoj koncentraciji. RF je pri dozama ≥ 20 µM uzrokovao statistički značajno (p< 0,001) smanjenje udjela stanica koje odumiru autofagijom u odnosu na kontrolni uzorak, što ukazuje na izmijenjenu biološku aktivnost od djelovanja samog RSV-a koji statistički značajno inducira autofagiju u CHO-K1 kulturi stanica.

Abstract (english)

In this study, the effect of resveratrol (RSV) and the ferrocene derivative of resveratrol (RF) (5, 20, 50 and 100 µM) on the induction of cell death in ovarian cell culture (CHO-K1 cell line) and keratinocytes (HaCaT cell line) was determined. In addition, viability and cell proliferation were monitored using the Neutral Red and Trypan Blue methods. After treatment of cells with RSV, lower IC values obtained by the Trypan Blue method compared to the Neutral Red test were recorded in both cell lines, which can be explained by an increase in the number and size of lysosomes. Treatment of HaCaT cells with RSV resulted in a slightly increased proportion of cells undergoing apoptosis compared to the control sample, whereas in CHO-K1 cell culture this effect was more pronounced and statistically significant (at doses ≥20 μM). RSV induced autophagy in both cell cultures, and the value of the autophagy induction ratio varied depending on the concentration applied. At doses ≥ 20 µM, RF caused a statistically significant (p< 0.001) decrease in the proportion of cells that die by autophagy compared to the control sample, indicating altered biological activity of RF compared to the action of RSV itself (which induced autophagy in CHO-K1 cell culture).

• Keywords: apoptoza; autofagija; resveratrol; ferocenski derivat resveratrola; vijabilnost stanica
• Keywords (english): apoptosis; autophagy; resveratrol; ferrocene derivative of resveratrol; cell viability
• Language: croatian
• URN:NBN: urn:nbn:hr:159:525338
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-06-27 09:44:46