Učinci ferocenskog analoga kurkumina na citotoksičnost induciranu polikloriranim bifenilima

master's thesis

• Title (english): Effects of ferrocene analogue of curcumin on cytotoxicity induced by polychlorinated biphenyls
• Author: Ružica Bajić
• Mentor: Teuta Murati (mentor)
• Mentor: Ivana Kmetič (pomoć pri izradi, neposredni voditelj)
• Mentor: Marina Miletić (pomoć pri izradi, neposredni voditelj)
• Committee member: Ivana Kmetič (predsjednik povjerenstva)
• Committee member: Irena Landeka Jurčević (član povjerenstva)
• Committee member: Igor Slivac (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2023-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Cilj rada je bio ispitati mogući protektivni učinak ferocenom modificiranog polifenola kurkumina (1 – 20 μM) na citotoksičnost induciranu kongenerima polikloriranih bifenila (PCB) – PCB 77 i PCB 153 (75 μM) u kulturi stanica ovarija (CHO (Chinese Hamster Ovary) – K1 stanična linija). U svrhu određivanja vijabilnosti i proliferacije stanica korištene su metode Neutral Red, Kenacid Blue i Trypan Blue, a primjenom fluorimetrijske metode detektiran je učinak na stvaranje reaktivnih kisikovih vrsta (ROS). Predtretman stanica ferocenskim analogom kurkumina nije pokazao značajan učinak na vijabilnost stanica tretiranih sa PCB 77, iako je pri određenim koncentracijama analoga određeno inhibitorno djelovanje. Prilikom predinkubacije stanica analogom, a potom tretiranih sa PCB 153 nije uočen učinak na proliferaciju i vijabilnost stanica što je potvrđeno primjenom svih triju metoda. Analog je u svim ispitanim koncentracijama pokazao antioksidacijski učinak, odnosno doveo do pada razine ROS u odnosu na stanice koje su tretirane samo sa PCB 153. Statistički značajan pad razine ROS uočen je kod predtretmana stanica analogom kurkumina u koncentracijama od 1 μM (p< 0,05), 2,5 μM (p< 0,025) i 20 μM (p< 0,005).

Abstract (english)

The aim of the work was to examine the possible protective effect of the ferrocene-modified polyphenol curcumin (1-20 μM) on cytotoxicity induced by congeners of polychlorinated biphenyls (PCBs) - PCB 77 and PCB 153 (75 μM) in the culture of ovarian cells (CHO (Chinese Hamster Ovary) - K1 cell line). Neutral Red, Kenacid Blue and Trypan Blue methods were used to determine cell viability and proliferation, and reactive oxygen species (ROS) formation was detected by the fluorimetric method. Pretreatment of cells with the ferrocene analogue showed no significant effect on the viability of cells treated with PCB 77, although an inhibitory effect was detected at certain analogue concentrations. No effect on cell proliferation and viability was observed when cells were preincubated with the analogue and then treated with PCB 153 (confirmed by all three methods). The analogue showed an antioxidant effect at all concentrations tested, i.e. it resulted in decrease in the level of ROS compared with cells treated with PCB 153 alone. A statistically significant decrease in ROS level was observed when cells were pretreated with curcumin analogue at concentration of 1 μM (p< 0.05), 2.5 μM (p< 0.025), and 20 μM (p< 0.005).

• Keywords: kurkumin; ferocen; PCB; citotoksičnost; ROS
• Keywords (english): curcumin; ferrocene; PCB; cytotoxicity; ROS
• Language: croatian
• URN:NBN: urn:nbn:hr:159:280433
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-07-01 11:57:03