Učinak prihrane CHO DP-12 stanica peptidnim frakcijama hidrolizata industrijske konoplje na stanični rast i produktivnost

master's thesis

• Title (english): The impact of hempseed protein hydrolsate feed on growth and productivity of CHO DP-12 cell line
• Author: Romana Ivković
• Mentor: Igor Slivac (mentor)
• Mentor: Marijan Logarušić (pomoć pri izradi, neposredni voditelj)
• Committee member: Višnja Gaurina Srček (predsjednik povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Committee member: Teuta Murati (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2023-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Životinjski serum standardna je dopuna medija za uzgoj životinjskih stanica, no zbog brojnih ograničenja posljednjih se nekoliko desetljeća biljni proteinski hidrolizati istražuju kao njemu odgovarajuća zamjena. Ovim je radom istražen učinak prihrane peptidnim frakcijama proteinskih hidrolizata konoplje na rast i produktivnost CHO-DP 12 stanica, proizvođača monoklonskog protutijela IgG. U ovom radu stanice su prihranjivane u kasnijoj fazi šaržnog uzgoja peptidnim frakcijama hidrolizata veličine < 10 kDa u koncentraciji od 2 g L⁻¹ dobivenih pomoću enzima Neutraza i Protamex. Ustanovljeno je da peptidna frakcija dobivena enzimom Neutraza inhibira rast stanica bilo da se dodaje kulturi za šaržni uzgoj ili kao prihrana, dok peptidna frakcija dobivena enzimom Protamex inhibira stanični rast u uvjetima šaržnog uzgoja, a kao prihrana nema nikakav ili ima pozitivan učinak na rast. Stanice uzgajane uz dodatak proteinskih hidrolizata pokazale su manju produktivnost od onih u kontrolnim uvjetima. Vrijeme prihrane utječe na jačinu opaženih učinaka na rast i produktivnost.

Abstract (english)

Animal serum is a common supplement in cell culture media, but due to various limitations, scientists are focused on finding suitable alternatives. Plant protein hydrolysates have recently been studied for this purpose. This study investigated the impact of hempseed protein hydrolysate feed on growth and productivity of CHO DP-12 cells. During batch cultivation, cells were fed with peptide fractions of < 10 kDa at a concentration of 2 g L⁻¹ , obtained using the enzymes Neutrase and Protamex. It was found that peptide fraction obtained using Neutrase inhibited cell growth, in both batch and fed-batch mode, while peptide fraction obtained using Protamex inhibited cell growth in batch culture conditions but had either no effect or a positive effect as a feed. Cells cultured with the addition of protein hydrolysates showed lower productivity compared to the control conditions. The timing of supplementation influenced the magnitude of the observed effects on growth and productivity.

• Keywords: CHO DP-12 stanična linija; proteinski hidrolizati; konoplja; šaržni uzgoj s prihranom; protutijelo IgG
• Keywords (english): CHO DP-12 cell line; protein hydrolisates; hempseed; fed-batch; antibody IgG
• Language: croatian
• URN:NBN: urn:nbn:hr:159:968888
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-07-09 11:14:39