Proizvodnja suhih aktivnih pripravaka probiotičkih bakterija liofilizacijom

undergraduate thesis

• Title (english): Production of active probiotic cultures as freeze-dried formulations
• Author: Helena Dragić
• Mentor: Jasna Novak (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2014-09-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Cilj ovog rada je proizvodnja funkcionalnih starter kultura u liofiliziranom obliku s ciljem ispitivanja mogućnosti ovih bakterija da zadrže aktivnost tijekom skladištenja. U tu svrhu određeno je preživljenje različitih probiotičkih sojeva bakterija mliječne kiseline, tijekom procesa liofilizacije. U ovom radu provedena je liofilizacija 12 različitih sojeva bakterija mliječne kiseline, 11 iz roda Lactobacillus i jednog soja iz roda Bifidobacterium uz primjenu obranog mlijeka kao lioprotektora. Prije i nakon liofilizacije određen je, indirektnom metodom, postotak preživljavanja ispitivanih probiotičkih bakterija. Iz rezultata proizlazi, da su svi sojevi zadovoljili minimalan broj živih stanica propisan za probiotičke pripravke, odnosno da broj preživjelih bakterijskih stanica iznosi više od 106 CFU/ml, što nam dokazuje da se obrano mlijeko pokazalo kao prikladan medij, odnosno lioprotektor za liofilizaciju probiotičkih kultura.

Abstract (english)

The aim of this work is the production of probiotic cultures in freeze-dried form, to verify whether these strains can apprehend activity during their storage, by determining their cell viability, during freeze-drying. In this work, freeze-drying of the 12 different strains: 11 strains of Lactobacillus genus and one of Bifidobacterium genus, was conducted by using skim milk as a lyoprotecant. Before and after freeze-drying the survival rate of tested probiotic strains was determined, with an indirect method. The results showed that for all strains the number of the survived cells was greater than 106 CFU/ml suggesting that all strains satisfied minimal number of the viable cells requested for probiotic formulations. In addition, overall results pointed out that skimmed milk is an appropriate media i.e. lyoprotectan for the lyophilisation of tested probiotic cultures.

• Keywords: liofilizacija; lioprotektori; probiotici
• Keywords (english): freeze-drying; probiotics; lyoprotectans
• Language: croatian
• URN:NBN: urn:nbn:hr:159:916467
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 13:34:56