Procjena protokola za pripremu uzoraka krvne plazme za određivanje butirata GC/MS metodom

undergraduate thesis

• Title (english): Evaluation of the protocols for the preparation of bllod plasma samples for determination of butyrate by the GC/MS method
• Author: Tara Krpan
• Mentor: Jurica Žučko (mentor)
• Mentor: Andrijana Meščić Macan (pomoć pri izradi, neposredni voditelj)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2024-09-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu ispitivala se učinkovitost pripreme uzoraka krvne plazme za određivanje koncentracije butirata prema dva protokola. Koncentracija butirata indikator je zdravlja organizma, a butirat je važan zbog mnogih pozitivnih učinaka koje ima na ljudski organizam. Provedena su dva protokola pripreme uzorka i na kraju se uspoređivala njihova pogodnost za daljnju analizu na uređaju za GC/MS. Kao re zultat dobiveno je da su oba protokola dala zadovoljavajuće odvajanje faza i ekstrakciju kratkolanča nih masnih kiselina, ali za daljnju analizu ostalih uzoraka koristio bi se Protokol 1 zbog preciznijeg od vajanja faza i manje mogućnosti kontaminacije talogom.

Abstract (english)

In this study, we compared the efficiency of two protocols for preparing blood plasma samples for bu tyrate concentration determination using gas chromatography-mass spectrometry (GC/MS). Butyrate is a crucial biomarker of health due to its numerous beneficial effects on the human body. Both proto cols achieved satisfactory phase separation and extraction of short-chain fatty acids. However, Proto col 1 exhibited superior performance, demonstrating more precise phase separation and a reduced risk of sediment contamination. Therefore, Protocol 1 is recommended for future sample preparation in this context to ensure accurate and reliable butyrate concentration measurements using GC/MS. Keywords: butyric acid, GC/MS analysis, phase separation

• Keywords: butirat; GC/MS; odvajanje faza
• Keywords (english): butyric acid; GC/MS analysis; phase separation
• Language: croatian
• URN:NBN: urn:nbn:hr:159:250786
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-09-25 10:11:23