Određivanje genotoksičnog učinka glifosata, kaptana, spinosada i deltametrina na humanim kontinuiranim staničnim linijama karcinoma želuca i jetre

master's thesis

• Title (english): Determination of the genotoxic effect of glyphosate, captan, spinosad and deltametrin on human continuous cell lines of stomach and liver carcinoma
• Author: Fiona Shahini
• Mentor: Ksenija Durgo (mentor)
• Mentor: Ana Huđek Turković (pomoć pri izradi, neposredni voditelj)
• Committee member: Marina Krpan (predsjednik povjerenstva)
• Committee member: Nevenka Kopjar (član povjerenstva)
• Committee member: Kristina Radošević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2024-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Pesticidi su tvari koje se upotrebljavaju za uništavanje ili kontrolu štetnih organizama, uključujući nepoželjne kukce, životinje i biljke te organizme koji prenose bolesti. Zbog masovne upotrebe pesticida i izloženosti opće populacije bitno je istražiti njihove potencijalne toksične učinke. U ovom su radu testirana četiri pesticida: herbicid glifosat, fungicid kaptan te insekticidi spinosad i deltametrin pri koncentracijama koje su okolišno značajne i koje se mogu naći u vodi, tlu, zraku, kao i u tretiranoj hrani. Ispitan je njihov citotoksični, proliferacijski, prooksidacijski i genotoksični učinak na kontinuiranim humanim staničnim linijama adenokarcinoma epitela želuca- AGS i hepatocelularnog karcinoma jetre- Hep G2. Dobiveni rezultati pokazali su da niti jedan testirani pesticid ne djeluje citotoksično ni prooksidacijski na ispitivanim staničnim linijama. Testirani pesticidi ne uzrokuju promjene u proliferativnoj sposobnosti stanica, međutim glifosat i kaptan pri najvišim koncentracijama pokazuju inhibitorni učinak na staničnu diobu. Samo je glifosat pokazao povećanje parametara genotoksičnih učinaka, ukazujući na potencijalno genotoksično djelovanje.

Abstract (english)

Pesticides are substances used to destroy or control harmful organisms, including unwanted insects, animals, plants, and disease-transmitting organisms. Due to the widespread use of pesticides and their exposure to the general population, it is essential to investigate their potential toxic effects. This study tested four pesticides: the herbicide glyphosate, the fungicide captan, and the insecticides spinosad and deltamethrin, at environmentally relevant concentrations found in water, soil, air, and treated food. Their cytotoxic, proliferative, pro-oxidative, and genotoxic effect was examined on continuous human cell lines of gastric epithelium adenocarcinoma- AGS and liver hepatocellular carcinoma- Hep G2. The results showed that none of the tested pesticides exhibited cytotoxic or pro-oxidative effects on the examined cell lines. The tested pesticides did not alter cell proliferative capacity, although glyphosate and captan at the highest concentrations showed an inhibitory effect on cell division. Only glyphosate showed an increase in genotoxic parameters, indicating a potential genotoxic effect.

• Keywords: pesticidi; stanične linije AGS i Hep G2; genotoksičnost; citotoksičnost; prooksidacijsko djelovanje
• Keywords (english): pesticides; AGS and Hep G2 cell lines; genotoxicity; cytotoxicity; prooxidative effect
• Language: croatian
• URN:NBN: urn:nbn:hr:159:629873
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-17 10:57:38