Karakterizacija bakterijske nanoceluloze dobivene fermentacijom kombuche u kemijski definiranim podlogama i podlogama s dodatkom kore lubenice

master's thesis

• Title (english): Characterization of bacterial nanocellulose obtained by fermentation of kombucha in chemically defined media and media with the addition of watermelon rind
• Author: Elena Klarić
• Mentor: Sunčica Beluhan (mentor)
• Committee member: Ksenija Markov (predsjednik povjerenstva)
• Committee member: Mirela Ivančić Šantek (član povjerenstva)
• Committee member: Blaženka Kos (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2024-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bakterijska nanoceluloza (BNC), u usporedbi s biljnom celulozom, predstavlja relativno čisti oblik celuloze koji posjeduje određena jedinstvena i superiorna strukturna, fizikalno-kemijska i mehanička svojstva. Iako posjeduju identičnu molekularnu strukturu, BNC ima bolju biokompatibilnost, poroznost, čistoću, polimerizaciju, vlačnu čvrstoću, kapacitet zadržavanja vode i mogućnost reciklacije (biorazgradivost). Komagateibacter xylinus uzgajana je na 3 standardne Hestrin- Schramm (HS) podloge u promijenjenom sastavu ugljika; glukoza, fruktoza i saharoza te na kompleksnoj podlozi od kore lubenice (KL). Debljina i prinos BNC povećavala se tijekom fermentacije, ovisno o površini i dubini hranjive podloge. Morfološke strukture BNC pelikula analizirane su pomoću skenirajuće elektronske mikroskopije (SEM). Uzorci su analizirani FT-IR spektroskopijom te je utvrđeno da su svi imali visoki postotak kristaliničnosti. WHC BNC sintetizirane u KL podlozi pokazao je najveću sposobnost zadržavanja vode dok su vrijednosti WRR nakon 72 sata bile potpuno jednake za BNC iz HS-Glc i HS Sah, a niže za BNC iz HS-Fru i KL.

Abstract (english)

Bacterial nanocellulose (BNC), compared to plant cellulose, represents a relatively pure form of cellulose that possesses certain unique and superior structural, psycho- chemical and mechanical properties. Although they have an identical molecular structure, BNC has better biocompatibility, porosity, purity, polymerization ability, tensile strength, water retention capacity and recyclability (biodegradability). Komagateibacter xylinus was grown on 3 standard Hestrin-Schramm (HS) mediums prepared in a changed carbon composition; glucose, fructose and sucrose as well as on a complex medium obtained from watermelon rind (WR). The thickness and yield of BNC increased during fermentation, depending on the surface and depth of the nutrient medium. The morphological structures of the produced BNC pellicles were analyzed using scanning electron microscopy (SEM). The samples were analyzed by FT-IR spectroscopy and it was determined that they all had a high percentage of crystallinity. WHC BNC synthesized in WR substrate showed the highest water retention capacity, while WRR values, after 72 hours, were exactly the same for BNC from HS-Glc and HS-Sah, and lower for BNC from HS-Fru and WR.

• Keywords: bakterijska nanoceluloza (BNC); agroindustrijski otpadci; fermentacija; kora lubenice
• Keywords (english): bacterial nanocellulose (BNC); agro-industrial waste; fermentation; watermelon rind
• Language: croatian
• URN:NBN: urn:nbn:hr:159:009034
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-10-17 12:08:29