Analiza genetičke raznolikosti i proteomička karakterizacija bakterija mliječne kiseline izoliranih iz rasola kiselog kupusa

undergraduate thesis

• Title (english): Analysis of genetic diversity and proteomic characterization of lactic acid bacteria isolated from sauerkraut brine
• Author: Tina Melkić
• Mentor: Martina Banić (mentor)
• Mentor: Katarina Butorac (pomoć pri izradi, neposredni voditelj)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology); Zagreb
• Defense date and country: 2024-06-17, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Bakterije mliječne kiseline (BMK) naseljavaju različite dijelove ljudskog tijela i ekosustave poput biljaka, tla, mlijeka i mesa. Komercijalno se primjenjuju u prehrambenoj, kozmetičkoj, farmaceutskoj i kemijskoj industriji. U ovom radu izolirano je 30 sojeva BMK iz rasola tradicionalno proizvedenog kiselog kupusa. Izmjerene koncentracije DNA navedenih sojeva oscilirale su u rasponu od 89,19 ng/μL do 475,96 ng/μL, s prosječnom vrijednošću od 238,77 ± 89,18 ng/μL. Provedeno je genotipsko profiliranje pomoću RAPD-PCR analize te su dobiveni elektroforetski profili RAPD-PCR produkata uspoređeni hijerarhijskom klaster analizom s ciljem eliminacije istovjetnih bakterijskih izolata. Analizom dendrograma, ustanovljeno je da je 30 sojeva svrstano u 3 klastera, što ukazuje da su iz rasola kiselog kupusa izolirana tri genetički različita soja. SDS-PAGE analizom nije utvrđena prisutnost dominantnih proteinskih vrpci veličine oko 50 kDa, što ukazuje da sojevi izolirani u ovom radu najvjerojatnije ne proizvode S-proteine.

Abstract (english)

Lactic acid bacteria (LAB) inhabit various parts of the human body and ecosystems such as plants, soil, milk, and meat. They are commercially utilised in the food, cosmetic, pharmaceutical and chemical industries. In this work, 30 LAB strains were isolated from the brine of traditionally produced sauerkraut. The measured DNA concentrations of these strains ranged from 89.19 ng/μL to 475.96 ng/μL, with an average value of 238.77 ± 89.18 ng/μL. Genotypic profiling was performed using RAPD-PCR analysis and the obtained electrophoretic profiles of the RAPD-PCR products were compared using hierarchical cluster analysis to eliminate identical bacterial isolates. Dendrogram analysis revealed that 30 strains were divided into 3 clusters, indicating that three genetically distinct strains were isolated from the sauerkraut brine. SDS-PAGE analysis revealed no dominant protein bands with a molecular weight of about 50 kDa, indicating that the strains isolated in this work most likely do not produce S-proteins.

• Keywords: bakterije mliječne kiseline; RAPD-PCR; SDS-PAGE; hijerarhijska klaster analiza
• Keywords (english): lactic acid bacteria; RAPD-PCR; SDS-PAGE; hierarchical cluster analysis
• Language: croatian
• URN:NBN: urn:nbn:hr:159:275051
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-11-14 13:36:27