Izolacija, karakterizacija i fenolni profil bioaktivnih komponenti iz lista i ploda planike s područja Malog Lošinja

master's thesis

• Title (english): Isolation, characterization, and phenolic profile of bioactive compounds from leaves and fruits of strawberry tree from the region of Mali Lošinj
• Author: Nikolina Račić
• Mentor: Senka Djaković (mentor)
• Mentor: Jasmina Lapić (pomoć pri izradi, neposredni voditelj)
• Mentor: Anica Bebek Markovinović (pomoć pri izradi, neposredni voditelj)
• Committee member: Danijela Bursać Kovačević (član povjerenstva)
• Committee member: Martina Bituh (član povjerenstva)
• Committee member: Jasmina Lapić (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Organic Chemistry); Zagreb
• Defense date and country: 2024-11-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Obična planika samonikla je šumska vrsta iz porodice Ericaceae. U Hrvatskoj obuhvaća područje od Istre do Dubrovačkog primorja, uključujući otoke. Tradicionalno su plodovi, lišće i korijenje korišteni u medicinske svrhe budući da ova vrsta pokazuje farmakološka svojstva. U ovom radu izolirani su bioaktivni spojevi primjenom konvencionalne ekstrakcije grijanjem u povrat, ekstrakcije po Soxhlet-u i ultrazvučne ekstrakcije, uz destiliranu vodu, 70 %-tni etanol i etil-acetat kao ekstrakcijska otapala. Fenolni profil biljnih ekstrakta određen je spektrofotometrijskim određivanjem ukupnih fenola, hidroksicimetnih kiselina, flavonola i kondenziranih tanina, a antioksidacijski kapacitet lista i ploda određen je FRAP (engl. Ferric Reducing/Antioxidant Power) i ABTS (radikal 2,2'-azinobis (3etilbenzotiazolin-6-sulfonske kiseline)) metodama. Dobiveni ekstrakti pročišćeni su kolonskom kromatografijom i karakterizirani IR - spektroskopijom. Rezultati su pokazali kako je udio polifenola veći u ekstraktima lista, u odnosu na ekstrakte plodova, a konvencionalna ekstrakcija grijanjem u povrat uz 70 %-tni etanol kao ekstrakcijsko otapalo pokazala se najunčikovitijom za određivanje fenolnog profila.

Abstract (english)

The strawberry tree is a wild forest species from the Ericaceae family. In Croatia, it spans from Istria to the Dubrovnik coast, including the islands. Traditionally, its fruits, leaves, and roots have been used in herbal medicine due to the pharmacologicalproperties. This study focused on isolating bioactive compounds using conventional heat-reflux extraction, Soxhlet extraction, and ultrasound-assisted extraction, employing distilled water, 70% ethanol, and ethyl acetate as solvents. The phenolic profile of the plant extracts was measured spectrophotometrically, analyzing total phenols, hydroxycinnamic acids, flavonols, and condensed tannins. The antioxidant capacity of the leaves and fruits was evaluated using the FRAP (Ferric Reducing/Antioxidant Power) and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6sulphonic acid)) assays. The resulting extracts were further purified via column chromatography and characterized by IR spectroscopy. The results indicated that the polyphenol content was higher in leaf extracts compared to fruit extracts. Among the extraction methods, conventional reflux extraction using 70% ethanol as the solvent proved to be the most effective for profiling phenolic compounds.

• Keywords: Arbutus unedo L.; bioaktivne komponente; polifenoli; ekstrakcija; IR spektroskopija
• Keywords (english): Arbutus unedo L.; bioactive compounds; polyphenols; extraction; IR spectroscopy
• Language: croatian
• URN:NBN: urn:nbn:hr:159:842774
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2025-12-31
• Created on: 2024-12-03 14:43:05