Utjecaj rapamicina na životni vijek vinske mušice Drosphila melanogaster ovisan o životnoj dobi

master's thesis

• Title (english): Age-dependent effect of rapamycin on Drospophila melanogaster lifespan
• Author: Paula Juričić
• Mentor: Slobodan Barbarić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Biochemistry); Zagreb
• Defense date and country: 2013-12-06, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Brojne studije su pokazale da rapamicin može produljiti životni vijek mnogih organizama. Ciljevi ove studije bili su (i) ispitati utjecaj koncentracije proteina u mediju za uzgoj na duljinu životnog vijeka vinske mušice Drosophile melanogaster, (ii) usporediti utjecaj standardnog nedefiniranog i kemijskog definiranog medija na životni vijek te (iii) utvrditi može li tretman rapamicinom kasnije u životu pozitivno utjecati na životni vijek vinske mušice. Sukladno prethodnim, ovo istraživanje je pokazalo da povećana količina proteina u prehrani negativno utječe na životni vijek vinske mušice. Prema rezultatima, kratkotrajno izlaganje rapamicinu, u trajanju od četiri tjedna, produljuje životni vijek u približno istoj mjeri kao i cjeloživotni tretman. Uz to, rapamicin je smanjio plodnost vinske mušice ali nakon prestanka tretmana rapamicinom, plodnost je vraćena na normalnu razinu.

Abstract (english)

Recent studies demonstrated that chronic rapamycin treatment extends lifespan and reduces fecundity of fruit fly Drosophila melanogaster females. The aim of this thesis was (i) to investigate the effects of increased protein concentration in diet on longevity of the fruit fly, (ii) to compare the effects of standard and chemically defined medium on lifespan and (iii) to determine whether pharmacological intervention with rapamycin late in life have beneficial effects on fly survival. In accordance with others, this study showed that increased protein component of a diet decreases lifespan. It was found that even short rapamycin treatments, of about three weeks, can extend maximum lifespan of a fruit fly to the level comparable to the chronic rapamycin treatment. This effect was observed on all diets tested, suggesting that the effect of rapamycin is at least partially independent of nutrition. Additionally, rapamycin reduced fecundity of females and releasing flies from rapamycin treatment resulted in recovery of fecundity.

• Keywords: starenje; TOR signalni put; rapamycin; prehrana
• Keywords (english): ageing; TOR pathway; rapamycin; nutrition
• Language: croatian
• URN:NBN: urn:nbn:hr:159:644529
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 08:45:18