Biološko uklanjanje dušika i fosfora u anaerobno-aerobno-anoksičnom procesu uz endogenu denitrifikaciju iz otpadne vode niskoga omjera ugljika i dušika

doctoral thesis

• Title (english): Biological removal of nitrogen and phosphorus in an anaerobic-oxic-anoxic process with endogenous denitirfication from wastewater with a low carbon-to-nitrogen ratio
• Author: Tea Štefanac
• Mentor: Tibela Landeka Dragičević (mentor)
• Committee member: Dijana Grgas (predsjednik povjerenstva)
• Committee member: Ksenija Durgo (član povjerenstva)
• Committee member: Mirna Habuda-Stanić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for the Biological Waste Water Treatment); Zagreb
• Defense date and country: 2024, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

Aktivni mulj iz sustava za pročišćavanje otpadne vode, koji ne provodi napredno biološko uklanjanje fosfora, obogaćen je fosfat akumulirajućim organizmima. Anoksično-aerobno uklanjanje ugljika, dušika i fosfora uz natrijev acetat kao izvor ugljika i C/P 1, 3, 5, 7, 10, rezultiralo je odabirom duljine trajanja anoksične faze procesa od 2 sata, i u aerobnoj fazi daljnjom oksidacijom KPK, 51,9% uklanjanjem fosfora i potpunim uklanjanjem dušika. U anaerobno-aerobno-anoksičnom procesu uz endogenu denitrifikaciju uz natrijev acetat kao izvor ugljika postignuto je uklanjanje P od 78,5% i uklanjanje N od 76,6% (pri DO ≥2 mg/L), te uklanjanje P od 77,9% i uklanjanje N od 95,3% (pri DO ~1 mg/L). Endogena denitrifikacija je doprinijela uklanjanju NOx, te je uklonjeno 73% N, odnosno 97% N. FISH metodom dokazani su klasteri funkcionalnih mikroorganizama (AOB, NOB, DNI, PAOs/DPAOs, GAOs/DGAOs).

Abstract (english)

Activated sludge from wastewater treatment systems that do not conduct advanced biological phosphorus removal was enriched with phosphate-accumulating organisms. Anoxic-aerobic removal of carbon, nitrogen, and phosphorus, using sodium acetate as the carbon source and varying C/P ratios (1, 3, 5, 7, 10), resulted in the selection of a 2-hour anoxic phase duration. In the aerobic phase, further chemical oxygen demand (COD) oxidation was achieved, 51.9% phosphorus and complete nitrogen removed. In the anaerobic-aerobic-anoxic process with endogenous denitrification, using sodium acetate as the carbon source, phosphorus removal reached 78.5% and nitrogen removal 76.6% (at DO ≥ 2 mg/L), and phosphorus removal was 77.9% with nitrogen removal at 95.3% (at DO ~1 mg/L). Endogenous denitrification contributed to NOx removal, achieving nitrogen removal rates of 73% and 97%, respectively. Functional microorganism clusters (AOB, NOB, DNI, PAOs/DPAOs, GAOs/DGAOs) were confirmed using the FISH method.

• Keywords: acetat; anaerobno-aerobno-anoksični proces; DPAOs; endogena denitrifikacija; PAOs
• Keywords (english): acetate; anaerobic-oxic-anoxic process; denitrifying phosphate-accumulating organisms (DPAOs); endogenous denitrification; phosphate-accumulating organisms (PAOs)
• Language: croatian
• URN:NBN: urn:nbn:hr:159:971610
• Promotion: 2024
• Study programme: Title: Postgraduate PhD Study in Nutrition; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/ doktorica znanosti, područje biotehničkih znanosti, odgovarajuće polje (određeno matičnom strukom) (doktor/ doktorica znanosti, područje biotehničkih znanosti, odgovarajuće polje (određeno matičnom strukom))
• Type of resource: Text
• Extent: 134 str ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2024-12-31 10:58:52