Biokatalitička redukcija ugljikova dioksida primjenom niskotemperaturnih eutektičkih otapala

master's thesis

• Title (english): Biocatalytic reduction of carbon dioxide using deep eutectic solvents
• Author: Karla Šubar
• Mentor: Marina Cvjetko Bubalo (mentor)
• Mentor: Marijan Logarušić (pomoć pri izradi, neposredni voditelj)
• Committee member: Ana Jurinjak Tušek (predsjednik povjerenstva)
• Committee member: Ivana Radojčić Redovniković (član povjerenstva)
• Committee member: Davor Valinger (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2024-12-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Ovaj diplomski rad istražuje mogućnosti primjene niskotemperaturnih eutektičnih otapala (engl. Deep Eutectic Solvents, DES) u biokatalitičkoj redukciji CO₂ primjenom enzima formijat dehidrogenaze (FDH). Ispitana je aktivnost FDH, topljivost CO₂ te stabilnost koenzima u DES-ovima na bazi betaina i kolin-klorida s glicerolom, pri različitim udjelima vode odnosno pufera. Utvrđeno je da je aktivnost FDH veća i topljivost CO₂ bolja u DES-ovima s kolin-kloridom, dok udio vode odnosno pufera značajno utječe na aktivnost FDH, kratkoročnu stabilnost NADH i topljivost CO₂. Spearmanova korelacijska analiza je dokazala da struktura DES-ova te fizikalno-kemijska svojstva poput viskoznosti, pH i gustoće utječu na aktivnost FDH, kratkoročnu stabilnost NADH i topljivost CO₂. Matematički modeli za predikciju svojstava DES-ova, temeljeni na fizikalno-kemijskim svojstvima i sigma profilima, pokazali su se korisnim za industrijske primjene i istraživanja. Njihova učinkovitost potvrđena je redukcijom CO₂ u formijat kataliziranom FDH-om, što otvara nove mogućnosti za primjenu DES-ova u biokatalizi.

Abstract (english)

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This graduate thesis investigates the possibilities of using Deep Eutectic Solvents (DES) in the biocatalytic reduction of CO₂ using the enzyme formate dehydrogenase (FDH). FDH activity, CO₂ solubility and coenzymes stability were tested in DESs based on betaine and choline chloride with glycerol, at different proportions of water and buffer. Results showed higher FDH activity and better CO₂ solubility in DESs with choline chloride, while the proportion of water or buffer significantly affects the FDH activity, the NADH short-term stability and the CO₂ solubility. Spearman's correlation analysis proved that DESs structure and physico-chemical properties such as viscosity, pH and density influence the FDH activity, the NADH short-term stability and the CO₂ solubility. Mathematical models for the prediction of DES properties, based on physicochemical properties and sigma profiles, have proven useful for industrial applications and research. Their effectiveness was confirmed by the reduction of CO₂ to formate catalyzed by FDH, which opens new possibilities for the application of DES in biocatalysis.

• Keywords: niskotemperaturna eutektička otapala; biokataliza; formijat-dehidrogenaza; NADH; matematičko modeliranje
• Keywords (english): deep eutectic solvents; biocatalysis; formate dehydrogenase; NADH; mathematical modeling
• Language: croatian
• URN:NBN: urn:nbn:hr:159:659839
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-01-08 11:10:37