Raznolikost izolata roda Saprolegnia u hrvatskim pastrvskim uzgajalištima

master's thesis

• Title (english): Diversity of Saprolegnia isolates in Croatian trout farms
• Author: Ana Straga
• Mentor: Ana Bielen (mentor)
• Mentor: Dora Pavić (pomoć pri izradi, neposredni voditelj)
• Committee member: Lidija Šver (predsjednik povjerenstva)
• Committee member: Andreja Leboš-Pavunc (član povjerenstva)
• Committee member: Jurica Žučko (član povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2025-02-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom je radu metodom tipizacije višestrukih lokusa (engl. multilocus sequence typing, MLST) po prvi put analizirana genotipska raznolikost izolata roda Saprolegnia (S. parasitica, S. australis, S. delica i S. ferax) iz hrvatskih pastrvskih uzgajališta. Nađena je velika raznolikost genotipova (engl. diploid sequence type, DST), od kojih je većina bila opisana prvi put i specifična za lokaciju s koje je izolirana. Između pojedinih DST-ova iste vrste utvrđena je statistički značajna razlika u brzini radijalnog rasta micelija. Neki genotipovi (npr. DST7 vrste S. parasitica) nađeni su isključivo na životinjskom domaćinu, a drugi isključivo u vodi (npr. DST21 vrste S. parasitica). Temeljem toga može se pretpostaviti da neki genotipovi imaju veću virulenciju i predstavljaju veći rizik za pojavu od saprolegnioze u akvakulturnim postrojenjima.

Abstract (english)

In this study, the genotype diversity of Saprolegnia isolates (S. parasitica, S. australis, S. delica and S. ferax) from Croatian trout farms was analysed for the first time. The results of multilocus sequence typing (MLST) revealed a large diversity of genotypes (diploid sequence types, DSTs), most of which were described for the first time and were specific to the site where they were isolated. Significant differences in the mycelium growth rate were observed between different DSTs of the same species. Some genotypes (e.g. S. parasitica DST7) were found exclusively on the animal host, while others were found exclusively in water (e.g. S. parasitica DST21). Based on the results, it can be hypothesized that some genotypes have a higher virulence and therefore represent a higher risk for the occurrence of saprolegniosis in aquaculture systems.

• Keywords: MLST; genotipizacija; DST; saprolegnioza; pastrva
• Keywords (english): MLST; genotype; DST; trout; saprolegniosis
• Language: croatian
• URN:NBN: urn:nbn:hr:159:863561
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2025-02-28 09:13:13