Određivanje in vitro antioksidativnog kapaciteta, sadržaja polifenola i učinka na PMA-inducirano oksidativno izgaranje u ljudskim fagocitima odabranih ekstrakta voća

master's thesis

• Title (english): Assesment of the in vitro antioxidant capacity, phenolic content and of the effect on PMA-induced oxidative burst in human phagocytes of selected fruit extracts
• Author: Ana Jelekovac
• Mentor: Mauro Serafini (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Nutrition Science); Zagreb
• Defense date and country: 2014-02-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Namirnice biljnog podrijetla, osobito voće i povrće, smatraju se ključnim čimbenikom prehrambenih obrazaca sa pozitivnim učinkom na ljudsko zdravlje. Pozitivni učinak pripisuje se bioaktivnim vitaminima, mineralima, vlaknima i polifenolima. Višnja Maraska i limun, cijenjeno voće iz Dalmacije, kao i divlje jagode, smatraju se bogatim izvorom fitokemikalija. Stoga im je određen sadržaj ukupnih polifenola kao i neenzimatski antioksidativni kapacitet (NEAC), koji je određen dvijema različitim metodama; TRAP i FRAP. Učinak višnje Maraske i divljih jagoda na PMA-inducirano oksidativno izgaranje u fagocitima iz ex vivo ljudske plazme, određen je pomoću protočne citometrije. Divlje jagode su pokazale najviši udio polifenola i NEAC vrijednost, određenu FRAP-om, dok je višnja Maraska imala najveću NEAC vrijednost pri određivanju TRAP-om. Obje vrste voća pokazale su značajnu aktivnost u smanjivanju oksidativnog izgaranja u fagocitima, ističući veliku nutritivnu vrijednost i potencijal učinka na ljudsko zdravlje.

Abstract (english)

Plant food, especially fruits and vegetables, have been consistently identified as the main factor of dietary patterns beneficial for human health; this is attributed to the bioactivity of present vitamins, minerals, fibers and polyphenols. Sour cherry Maraska and lemon, appreciated fruits from Dalmatia, should be endowed with high polyphenol content, as well as wild type of strawberries. Thus, their total phenolic (TP) content was evaluated together with non enzymatic antioxidant capacity (NEAC), determined with two assays; total radical-trapping antioxidant parameter (TRAP) and ferric reducing-antioxidant power (FRAP). Effect of sour cherry Maraska and wild strawberry on PMA-induced oxidative burst in phagocytes from ex vivo human plasma was determined using flow cytometry. Wild strawberry displayed highest TP content and NEAC, when determined with FRAP, while sour cherry Maraska displayed highest NEAC values determined with TRAP. Both fruits displayed activity against PMA-induced oxidative burst what highlights nutritional value and health potentiality of this fruits.

• Keywords: oksidativno izgaranje; NEAC; polifenoli; višnja Maraska; divlje jagode
• Keywords (english): oxidative burst; NEAC; polyphenols; sour cherry Maraska; wild strawberry
• Language: croatian
• URN:NBN: urn:nbn:hr:159:892731
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 11:22:25