Ispitivanje citotoksičnih učinaka prirodnih ionskih kapljevina i eutektičkih otapala na CCO staničnoj liniji i kvascu Saccharomyces cerevisiae

master's thesis

• Title (english): Evaluation of cytotoxic effects of natural ionic liquids and deep eutectic solvents using CCO fish cell line and yeast Saccharomyces cerevisiae
• Author: Jelena Železnjak
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2014-09-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Uspostavljanje novih visokoučinkovitih i održivih procesa proizvodnje industrijski važnih kemikalija predmet je mnogih znanstvenih istraživanja u području kemijske tehnologije i biotehnologije. Odnedavno, prirodnim ionskim kapljevinama i eutektičkim otapalima pridaje se sve veća pozornost zbog zanemarivog tlaka pare. S obzirom na polarnost i selektivnost, one se mogu pripraviti za različite namjene kao što su kemijske ili enzimske reakcije te na taj način mogu zamijeniti agresivna i hlapljiva organska otapala. U ovom radu ispitana je in vitro toksičnost kolinijevih prirodnih ionskih kapljevina i eutektičkih otapala temeljenih na amino/organskim kiselinama kao anionima, odnosno donorima vodika na ribljoj staničnoj liniji CCO i kvascu Saccharomyces cerevisiae. Na temelju izračunatih EC50 vrijednosti toksični učinak navedenih otapala na CCO stanice najizraženiji je kod kolin-klorida:cinkova klorida (ChCl:ZnCl2) (umjereno citotoksičan) te kod glukoze:1-metilimidazola (Glc:1-MEI) (niska citotoksičnost), dok su se kod kvasca S. cerevisiae kao najtoksičnija otapala pokazala kolin-klorid:oksalna kiselina (ChCl:OA) i glukoza:1-metilimidazol (Glc:1-MEI).

Abstract (english)

Establishment of new highly productive and sustainable processes for the production of industrially important compounds is becoming a growing area of research in the field of chemical technology and biotechnology. Recently, bio ionic liquids (bio-ILs) and deep eutectic solvents (DES) have received increasing attention because they have a negligible vapor pressure and can be tailored based on their polarity and selectivity for different applications such as chemical or enzymatic reactions, thus, replacing current harsh organic solvents. This paper presents the results of toxic effects of selected choline-based bio-ILS and DES on the proliferation of CCO fish cell line and yeast Saccharomyces cerevisiae. Based on the calculated EC50 values, the most evident toxic effect on CCO cells was shown in choline chloride:zinc chloride (ChCl:ZnCl2) (moderate cytotoxicity) and glucose:1-methylimidazole (Glc:1-MEI) (low cytotoxicity), respectively choline chloride:oxalic acid (ChCl:OA) and glucose:1-methylimidazole (Glc:1-MEI) on S. cerevisiae.

• Keywords: prirodne ionske kapljevine; eutektička otapala; citotoksičnost; stanična linija CCO; Saccharomyces cerevisiae
• Keywords (english): bio-ionic liquids; deep eutectic solvents; cytotoxicity; CCO cell line; yeast Saccharomyces cerevisiae
• Language: croatian
• URN:NBN: urn:nbn:hr:159:536013
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 08:04:40