Optimiranje oksido-reduktivnih bioprocesa razgradnje bojila

master's thesis

• Title (english): Optimization of oxido-reductive bioprocesses of dyes degradation
• Author: Martina Kolarić
• Mentor: Tonči Rezić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2014-09-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Provedeno je istraživanje optimiranje parametara u procesu obezbojavanja bojila Direct blue 78 pomoću enzima lakaze u mikroreaktorskim sustavima, te bojila Direct blue 78, Basic blue 5, Basic red 46 i Basic blue 1 vodikovim peroksidom koristeći dizajn eksperimenta. Koncentracija apsorbiranog kisika mjerena u sustavu s mikroreaktorom pokazuje linearno povećanje s povećanjem protoka te je ostvarena vrijednost od 1,2 mM O2 min-1 pri protoku od 1,5 mL min-1. Obezbojavanje bojila Direct blue 78 u mikroreaktoru sa i bez aeracije pomoću enzima lakaze uzrokuje pad brzine obezbojenja od 50%, dok se u cijevnom mikroreaktoru s aeracijom povećanjem protoka brzina obezbojenja eksponencijalno smanjila za 80%. U cijevnom mikroreaktoru bez aeracije brzina obezbojavanja ostaje gotovo nepromijenjena bez obzira na povećanje protoka. Dizajnom eksperimenta najbolje je modeliran proces obezbojavanja bojila Basic blue 5, te je relativno zadovoljavajuće modeliranje procesa obezbojavanja ostvareno za bojila Basic blue 1.

Abstract (english)

In this reasearch, the optimization of parameters in the process of degradation of dyes Direct Blue 78, by the enzyme laccase in microreactors systems, and dyes Direct Blue 78, Basic Blue 5, Basic red 46 and Basic Blue 1 by hydrogen peroxide using design of experiment was performed. The concentration of adsorbed oxygen was measured in the microreactor system and it showed linear increase with the increaase in flow, and the value of 1,2 mM O2 min-1, with flow of 1,5 mL min-1, was achieved. Decolourisation of the Direct Blue 78 dye in a microreactor with and without aeration using laccase enzyme caused a 50% drop in speed of the decolourisation, whereas in the tubular microreactor with aeration increasing the flow velocity of the decolourisation exponentially decreased by 80%. In the tubular microreactor without aeration, regardless on the increased flow of the system speed, decolorization rate remained almost unchanged. The best modeled proces by experimental design was decolorization of Basic blue 5 dye, while relatively satisfactory was modeled proces of Basic blue 1 dye decolorization.

• Keywords: bojilo; mikroreaktor; lakaza; peroksidaza hrena; obezbojenje; dizajn eksperimenta
• Keywords (english): dyes; microreactor; laccase; horseradish peroxidase; discoloration; design of experiments
• Language: croatian
• URN:NBN: urn:nbn:hr:159:323084
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 08:35:10