Razvoj metalopeptida kao katalizatora

master's thesis

• Title (english): Developing metallopeptides for catalytic applications
• Author: Zrinka Aljinović
• Mentor: Lidija Barišić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Organic Chemistry); Zagreb
• Defense date and country: 2015-04-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Mali peptidi koji na svoje bočne ogranke mogu vezati Cu2+ ione pri pH-vrijednostima blizu neutralnih imaju veliki katalitički potencijal. U recentnom istraživanju Iranzo i suradnici sintetizirali su dva peptida, C-Asp i O-Asp, koji pri pH vrijednosti blizu neutralne vežu Cu2+ ione na svoja tri His i jedan Asp aminokiselinski ostatak tvoreći komplekse CuH(C-Asp)]2+ i [CuH(O-Asp)]2+. U ovom je radu sintetiziran novi OdPro-Asp dekapeptid čija je sposobnost koordiniranja Cu2+ iona pri pH-vrijednostima između 5 i 7 proučavana primjenom spektroskopskih tehnika. OdPro-Asp tvori kompleks [CuH(OdPro-Asp)]2+ koji veže Cu2+ ione na isti način kao ranije opisani kompleksi [CuH(C-Asp)]2+ i [CuH(O-Asp)]2+.Mogućnost primjene kompleksâ [CuH(C-Asp)]2+, [CuH(O-Asp)]2+ i [CuH(OdPro-Asp)]2+ kao katalizatora proučavana je korištenjem kvercetina i 3,4-dihidoksibenzojeve kiseline kao supstrata. Pokazalo se da ispitani kompleksi pri pH 6.2 kataliziraju reakcije s kvercetinom, dok katalitička uloga u reakcijama s 3,4-dihidroksibenzojevom kiselinom nije opažena.

Abstract (english)

Short peptides that can bind Cu2+ ions through the side-chain functionalities and generate single species in the neutral pH range may be capable of performing numerous catalytic activities. Recently, Iranzo and co-workers developed two peptides, C-Asp and O-Asp, which bind Cu2+ only to the 3 His and 1 Asp residues at close to neutral pH forming the complexes [CuH(C-Asp)]2+ and [CuH(O-Asp)]2+. Herein, the new decapeptide OdPro-Asp was synthesised and its Cu2+ coordination properties studied using different spectroscopic techniques. The major copper(II) species observed for the OdPro-Asp peptide between pH 5.0 and 7.0 can be described as [CuH(OdPro-Asp)]2+. This species coordinates Cu2+ ions in the same way as the complexes [CuH(C-Asp)]2+ and [CuH(O-Asp)]2+. The possibility of applying the complexes [CuH(C-Asp)]2+, [CuH(O-Asp)]2+ and [CuH(OdPro-Asp)]2+ as catalysts was studied using quercetin and 3,4-dihydroxide benzoic acid as substrates. The complexes reacted with quercetin, but did not react with 3,4-dihidroxide benzoc acid at pH 6.2.

• Keywords: sinteza; peptidi; bakar; koordinacija; kataliza
• Keywords (english): synthesis; peptides; copper; coordination modes; catalysis
• Language: english
• URN:NBN: urn:nbn:hr:159:421209
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 10:14:08