Redukcija etil-acetoacetata u etil-hidroksibutanoat pomoću kvasca Saccharomyces cerevisiae u eutektičnim otapalima

master's thesis

• Title (english): Reduction of ethyl-acetoacetate in ethyl-3-hydroxybutanoate with Saccharomyces cerevisiae in deep eutentic solvents
• Author: Ivana Lacković
• Mentor: Ivana Radojčić Redovniković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2014-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Posljednjih nekoliko godina eutektična se otapala intenzivno proučavaju kao zelena zamjena za sveprisutna tradicionalna škodljiva otapala u procesima organske sinteze i biokatalize te stoga privlače znatnu pozornost unutar biotehnološke i kemijske industrije. Biokataliza se pokazala kao ekološki prihvatljiva alternativa kemijskim katalizama, a posebna pozornost se posvećuje biokatalizi u nevodenim otapalima. U okviru ovog rada ispitana je redukcija etil-acetoacetata u etil-3-hidroksibutanoat u... More različitim eutektičnim otapalima primjenom pekarskog kvasca Saccharomyces cerevisiae kao biokatalizatora. Kolinijevi DES-ovi sa mnosaharidima (ksiloza, fruktoza i glukoza) i sa alkoholom glicerolom su se pokazali kao dobra zelena otapala, dok su u ostalim ispitanim DES-ovima dobivena mala iskorištenja. Less

Abstract (english)

In recent years, deep eutectic solvents have been intensively studied as a possible replacement for conventional molecular solvents for biocatalytic and organic reactions and have been the subject of considerable attention within chemical technology and biotechnology. Biocatalysis proved as environment friendly alternative to chemical catalysis and special attention has been devoted to biocatalysis in non-aqueous medium.Within this work, reduction of ethyl-acetoacetate to... More ethyl-3-hydroxybutanoate has been tested with a use of baker’s yeast Saccharomyces cerevisiae as a biocatalyst in deep eutectic solvents. DES with monosaccharides (xylose, fructose and glucose) and alcohol glycerol proved to be good green solvents, while in the other tested DES obtainded small yields. Less

• Keywords: biokataliza; etil-acetoacetat; eutektična otapala; redukcija; Saccharomyces cerevisiae
• Keywords (english): biocatalysis; deep eutectic solvents; ethyl-acetoacetate; reduction; Saccharomyces cerevisiae
• Language: croatian
• URN:NBN: urn:nbn:hr:159:289376
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 10:23:21