Proizvodnja vitamina B12 pomoću Pseudomonas denitrificans uz prihranjivanje betainom

undergraduate thesis

• Title (english): Production of vitamin B12 by Pseudomonas denitrificans with betaine feeding
• Author: Emina Nušinović
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2015-09-11, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Interdisciplinary Technical Sciences

Abstract

Vitamin B12 je crven higroskopan kristaličan prah, bez okusa i mirisa, topljiv u vodi i alkoholu. Pripada skupini korinoida, a danas se najčešće za vitamin B12 koristi naziv cijanokobalamin. Za razliku od drugih vitamina topljivih u vodi, vitamin B12 se pohranjuje i
može se dugo zadržati u našem organizmu. Važan je faktor rasta, ima ulogu u regeneraciji folata, metabolizmu aminokiselina, proteina i masti, važan je i za normalno funkcioniranje živčanog sustava, a njegov nedostatak može uzrokovati makrocitnu anemiju. Vitamin B12 proizvode mikroorganizmi (Propionibacterium freudenreichii i Pseudomonas denitrificans), a glavni izvor ovog vitamina su namirnice životinjskog podrijetla. S obzirom da se betain pokazao kao važan spoj za biosintezu vitamina B12, u ovom radu su prikazani rezultati proizvodnje vitamina B12 pomoću Pseudomonas denitrificans uz prihranjivanje betainom. Na osnovu rezultata određivanja učinaka tri ispitana raspona koncentracija betaina, razvijen je pristup prihranjivanja betainom u rasponu 5-7 g/L tijekom 50-140 sati fermentacije, što se pokazalo učinkovitim i ekonomičnim za proizvodnju vitamina B12 pomoću Pseudomonas denitrificans u fermentoru volumena 120m³.

Abstract (english)

Vitamin B12 is red hygroscopic crystalline powder that is tasteless, odourless, and soluble in water and alcohol. It belongs to a group of compounds called corinoids and the most common name used to denote vitamin B12 is cyanocobalamin. Unlike other watersoluble vitamins, vitamin B12 is stored and may stay in the body for long periods of time. It is an important growth factor, plays a role in the regeneration of folate and in the metabolism of amino acids, proteins and fats, it is important for the normal functioning of the nervous system, and its deficiency can cause macrocytic anemia. Vitamin B12 is produced by the microorganisms (Propionibacterium freudenreichii and Pseudomonas denitrificans), and the main source of this vitamin are foods of animal origin. Since betaine has been showed as an important compound in the biosynthesis of vitaminB12, this paper presents the results of the production of vitamin B12 by Pseudomonas denitrificans with betaine feeding. Based on the results of the determination of the effects of the three tested betaine concentration ranges, betaine feeding approach was developed, in the range 5-7 g /L for 50-140 hours of fermentation, which proved to be efficient and cost-effective for the
production of vitamin B12 by Pseudomonas denitrificans in a 120-m3 fementer.

• Keywords: vitaminB12; Pseudomonas denitrificans; betain; prihranjivanje
• Keywords (english): vitamin B12; Pseudomonas denitrificans; betaine; feeding
• Language: croatian
• URN:NBN: urn:nbn:hr:159:730517
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-02-08 12:17:12