Određivanje fuzarijskih mikotoksina ELISA metodom

master's thesis

• Title: Određivanje fuzarijskih mikotoksina ELISA metodom : DON, zearalenon, fuzarijski toksini, mikotoksikoze
• Title (english): Determination of fusarium mycotoxins using ELISA method
• Author: Tonka Kvočić
• Mentor: Ksenija Markov (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Process Engineering); (Section for Fundamental Engineering); Zagreb
• Defense date and country: 2015-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kontaminacija hrane i hrane za ţivotinje mikotoksinima značajan je svjetski problem. Mikotoksini su sekundarni metaboliti plijesni koji imaju negativne učinke na zdravlje ljudi, ţivotinja i usijeve rezultirajući bolestima i ekonomskim gubicima. Deoksinivalenol (DON) i zearalenon su mikotoksini sintetizirani od nekoliko vrsta plijesni roda Fusarium. U ovom istraţivanju,27 uzoraka kukuruza i 26 uzorka pšenice prikupljeni su upodručju istočne i sjeverozapadne Hrvatske tijekom 2014. godine. Koncentracije DON-a i zearalenona odreĎivane su primjenom validirane ELISA metode. Koncentracija DON-a premašuje najveće dopuštene vrijednosti u šest uzorka pšenice i sedam uzorka kukuruza, dok je koncentracija zearalenona veća od propisane izmjerena u tri uzorka pšenice i devet uzorka kukuruza. UsporeĎujući broj pozitivnih uzorka odnosno koncentracije analiziranih mikotoksina u pšenici i kukuruzu, kukuruz se pokazao kao više kontaminirana ţitarica. Visoke koncentracije DON-a i zearalenona u analiziranim ţitaricama mogu se povezati sa vremenskim uvjetima koji su 2014. godine okarakterizirani kao kišni do vrlo kišni te topli i vrlo topli.

Abstract (english)

The worldwide contamination of foods and feeds with mycotoxins is a significant problem. Mycotoxins are secondary metabolites of molds that have adverse effects on humans, animals and crops that result in illnesses and economic losses. Deoxynivalenol(DON) and zearalenone are mycotoxins produced by several species of mold which belong to the genus Fusarium. In this study, a total of 27 maize samples and 26 samples of wheat were collected from different fields situated in eastern and northwestern part of Croatia during 2014. Determination of DON and zearalenone concentration was done using validated ELISA (Enzyme-Linked Immunosorbent Assay) method. DON concentrations higher than allowed were observed in six wheat samples and seven maize samples while zearalenone concentration was higher than allowed in three wheat samples and nine maize samples. Comparing the number of positive samples and analyzed mycotoxin concentrations in wheat and maize, maize proved to be more contaminated cereal.High concentrations of DON and zearalenone in the analyzed cereals may be associated with weather conditions that characterized 2014 as a rainy to very rainy and warm to very warm.

• Keywords: DON; zearalenon; fuzarijski toksini; mikotoksikoze
• Keywords (english): DON; zearalenone; Fusarium mycotoxins; ELISA; mycotoxicosis
• Language: croatian
• URN:NBN: urn:nbn:hr:159:894393
• Study programme: Title: Bioprocess Engineering; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-04 10:04:26