Proizvodnja rekombinantnog proteina u CHO staničnoj liniji

undergraduate thesis

• Title (english): The production of recombinant proteins in CHO cell line
• Author: Karla Bojanić
• Mentor: Igor Slivac (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2016-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Proizvodnja rekombinantnih proteina u svrhu istraživanja i komercijalne upotrebe počima uspostavljanjem stanične linije. CHO stanična linija pogodna je za korištenje u tehologiji stanica jer se može genetički modificirati, uzgajati u suspenziji te raditi scale-up. Stanice mogu rasti u kemijskim definiranim medijima bez seruma i proteinskih hidrolizata. Cilj ovog rada je bio ispitati učinak različitih sastava medija za uzgoj na rast stanične linije, utrošak glukoze te proizvodnju proteina. Medij s 5 % seruma i 4% sojinog hidrolizata pokazao se kao najbolj za rast i prinos proteina, medij s 5% seruma bez sojinog hidrolizata je pokazao sporiji rast,dok je u mediju sa 1% seruma i 4% sojinog hidrolizata najsporiji rast što pokazuje da sojin hidrolizat nije dovoljno učinkovita zamjena za serum.

Abstract (english)

The production of recombinant proteins for research and commercial use begins by establishing a cell line. CHO cell line is suitable for use in technology of animal cells because cells can be genetically modified , grown in suspension , and the scale-up work. The cells may be grown in chemically defined medium without serum and protein hydrolysates . The purpose of this work was to examine the effect of different composition of culture medium on the growth of cell lines, the consumption of glucose and protein production. It was shown that the best medium for cells growth and protein yield was obtain with 5 % serum and 4 % soy hydrolyzate, medium with 5 % serum without soy hydrolyzate showed slower growth , while in the medium with 1 % serum and 4 % soy hydrolyzate resulted in the lowest cell growth and was shown that soy hydrolyzate is not sufficiently effective substitute for serum.

• Keywords: CHO stanična linija; hidrolizat soje; vijabilnost stanica
• Keywords (english): CHO cell line; soy hydrolysate; cell viability; SDS-PAGE
• Language: croatian
• URN:NBN: urn:nbn:hr:159:346190
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-21 12:16:41