Validacija metoda za određivanje mineralnih ulja u tlu FT-IR spektrometrijom i ukupnih masti i ulja ekstrakcijom

master's thesis

• Title (english): Validation of FT-IR method for the determination of mineral oils in soil and of extraction method for the determination of total oils and grease
• Author: Mia Barić
• Mentor: Vesna Zechner-Krpan (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2013-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Tlo je sastavni dio okoliša te se ubraja u obnovljive resurse s obzirom na dugotrajne procese nastanka i razvoja. Naftna industrija ima jedan od većih negativnih utjecaja kako na okoliš tako i na samo tlo. Česti izljevi nafte i naftnih derivata prilikom njihovog transporta i skladištenja dovode do onečišćenja tla mineralnim uljima i mastima. Cilj ovog rada bio je validirati metode za određivanje ukupnih i mineralnih masti i ulja u različitim uzorcima tla. Određivanje mineralnih ulja i ukupnih masti i ulja u uzorcima tla učinjeno je korištenjem dvaju različitih otapala. Metoda određivanja ukupnih masti i ulja Soxtec ekstrakcijom provedena je pomoću n-heksana, a metoda određivanja mineralnih masti i ulja sa tetrakloretilenom pomoću FT-IR spektroskopije. Metode su validirane i pokazale su zadovoljavajuće rezultate za uzorke iz okoliša. Metode su prihvatljive, jer se svi parametri validacije nalaze unutar granica prihvatljivosti prema American Association Of Official Analytical Chemisist (AOAC).

Abstract (english)

Soil is an integral part of the environment and is one of the renewable energy resources due to the long process of creation and development. The oil industry has a major negative impact on both the environment and the land itself. Frequent oil spills and oil products during their transportation and storage lead to pollution of soil mineral oils and greases. The aim of this study was to validate a method for the determination of total and mineral fats and oils in a variety of soil samples. Determination of mineral oil and total fats and oils in the soil samples was done using two different solvents. Method for determination of total fats and oils Soxtec extraction was performed using n-hexane, and a method for determining mineral fats and oils with tetrachlorethylene. Methods have been validated and shown satisfactory results for samples from the environment. Methods are acceptable, because all the validation parameters are within the limits of acceptability to the American Association of Official Analytical Chemisist (AOAC).

• Keywords: mineralna ulja; naftni ugljikovodici; tlo; onečišćenje; ekstrakcija
• Keywords (english): mineral oils; oil hydrocarbons; soil; contamination; extraction
• Language: croatian
• URN:NBN: urn:nbn:hr:159:911261
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-04 10:41:57