Određivanje citotoksičnog učinka pcb-a 153 i protektivnog učinka vitamina e metodom kenacid blue

master's thesis

• Title (english): Determination of cytotoxic effect of pcb 153 and protective effect of vitamin e by kenacid blue method
• Author: Tamara Novoselić
• Mentor: Branimir Šimić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2013-02-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu ispitan je učinak neplanarnog polikloriranog bifenila PCB-a 153 na proliferaciju CHO (Chinese Hamster Ovary)-K1 stanica metodom Kenacid Blue tijekom 72 sata. PCB 153 smatra se najrasprostranjenijim PCB kongenerom u biološkim sustavima. Karakterizira ga sposobnost nakupljanja u masnom tkivu živih organizama te otpornost na razgradnju u prirodnom okolišu zbog čega se bioakumulira u prehrambenom lancu. CHO-K1 stanice su u ovom istraživanju tretirane različitim koncentracijama PCB-a 153 (10-100 μM). Statistički značajna inhibicija stanične vijabilnosti utvrđena je nakon 24, 48 i 72 sata kod 75 μM i 100 μM PCB-a 153. IC50 vrijednosti za PCB 153 nakon 24, 48 i 72 sata tretmana iznose redom 97,13 μM, 62,17 μM i 67,48 μM. Ispitivanje mogućeg protektivnog učinka antioksidansa vitamina E na vijabilnost stanica tretiranih s 75 μM PCB-a 153 pokazuje njegovo blago protektivno djelovanje pri svim ispitanim koncentracijama (50, 75, 100 i 150 μM), no ono nije bilo statistički značajno.

Abstract (english)

In this study the effect of non-planar polychlorinated biphenyl 153 on the proliferation of CHO (Chinese Hamster Ovary)-K1 cells was examined using Kenacid Blue method over a period of 72 hours. PCB 153 is considered to be the most widespread PCB congener in biological systems. It has ability to accumulate in the fatty tissue of living organisms and the resistance to disintegration in the natural environment which is the reason why this congener bioaccumulates in the food chain. In this study CHO-K1 cells were treated with different concentrations of PCB 153 (10-100 μM). Statistically significant inhibition of cell viability was determined after 24, 48 and 72 hours at 75 μM and 100 μM of PCB 153. IC50 values for PCB 153 after 24, 48 and 72 hours are 97.13 μM, 62.17 μM and 67.48 μM. The study of possible impact of antioxidant vitamin E on the viability of cells treated with 75 μM PCB 153 showed its slight protective effect at all concentrations tested (50, 75, 100 and 150 μM), but the extent of effect was not statistically significant.

• Keywords: PCB 153; CHO-K1 stanična linija; citotoksičnost; vitamin E; Kenacid Blue metoda
• Keywords (english): PCB 153; CHO-K1 cell line; cytotoxicity; vitamin E; Kenacid Blue method
• Language: croatian
• URN:NBN: urn:nbn:hr:159:199536
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-04 11:55:26