Djelovanje ionskih tekućina na CCO stanice prilagođene na rast u mediju bez seruma

master's thesis

• Title (english): Effects of ionic liquids on CCO cells adapted to cell growth in the serum-free medium
• Author: Biljana Sipić
• Mentor: Višnja Gaurina Srček (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2012-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Posljednjih godina ionske tekućine zaokupljaju pažnju znanstvenika i industrije jer se koriste kao zamjena za hlapiva organska otapala (VOCs), no prije njihove šire primjene potrebno je odrediti moguću toksičnost U in vitro ekotoksikološkim istraživanjima te za ispitivanje utjecaja zagađenih voda na organizme koji žive u njoj najčešće se kao modelni sustavi koriste stanične linije riba. U istraživanju je korištena CCO (Channel Catfish Ovary) stanična linija prilagođena na rast u mediju bez seruma budući da na dostupnost ispitivanih tvari utječe niz fizikalno-kemijski čimbenika pa tako i sastav medija. U ovom radu prikazani su rezultati toksičnih učinaka odabranih ionskih tekućina na proliferaciju stanične linije CCO prilagođene na rast u mediju bez seruma (CCO-SF). Ispitan je učinak 4 različite ionske tekućine u koncentracijama od 0,1-10 mM, a vijabilnost stanica praćena je svaka 24 sata WST-1 metodom. EC50 vrijednosti izračunate nakon 72 sata od tretmana iznosile su: [C4MIM][PF6]=4,79 mM; [C4MIM][Tf2N] >10 mM; [C5MIM][Tf2N] >10 mM i [C10MIM][Tf2N] < 0,1 mM. Morfološke promjene inducirane djelovanjem ionskih tekućina na CCO-SF stanice u skladu su s zapaženom citotoksičnošću i ukazuju na promjene u gustoći i strukturi monosloja stanica.

Abstract (english)

Recently, ionic liquids occupy the attention of scientists and industry for usage as the replacements for volatile organic compounds (VOCs), but before their wider application it is necessary to determine their possible toxicity. Fish cell lines are used as model systems for in vitro ecotoxicological investigations and testing the impact of polluted water on the water organisms. In this study a CCO (Channel Catfish Ovary) cell line adapted to growth in serumfree conditions is used since the availability of substances is dependent upon number of physico-chemical factors, including the content of cultivation medium. This thesis presents the results of toxic effects of selected ionic liquids on the proliferation of CCO cell line adapted to growth in serum-free medium. The effect of four ionic liquids is tested in concentrations 0.1-10 mM, and cell viability was monitored every 24 hours by the WST-1 method. According to the obtained results EC50 values were calculated as follows: [C4MIM] [PF6]=4.79 mM, [C4MIM] [Tf2N]>10 mM, [C5MIM] [Tf2N]>10 mM and [C10M] [Tf2N] <0.1 mM. Morphological changes induced by ionic liquids in CCO-SF cells are in agreement with observed cytotoxicity and are seen as alternations in cell’s monolayer density and structure.

• Keywords: CCO stanična linija; medij bez seruma; ionske tekućine; citotoksičnost
• Keywords (english): CCO cell line; media without serum; ionic liquids; cytotoxicity
• Language: croatian
• URN:NBN: urn:nbn:hr:159:929499
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-09 09:12:16