Primjena kulture životinjskih stanica u ispitivanjima toksičnosti ksenobiotika

undergraduate thesis

• Title (english): Application of animal cell cultures in toxicity assessment of xenobiotics
• Author: Marina Miletić
• Mentor: Ivana Kmetič (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2010-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Kulture životinjskih stanica se koriste u industrijskoj biotehnologiji te u brojnim znanstvenim disciplinama, između ostalog i kao test sustavi u cilju ispitivanja toksičnosti ksenobiotika. U farmaceutskoj industriji njihova primjena vrlo je važna pri proizvodnji visokovrijednih pripravaka poput humanih i veterinarskih cijepiva, hormona, neuroregulatora i sl. Uključivanje in vitro metoda u toksikološka ispitivanja nužno je u cilju smanjenja broja životinja u pokusima. Osim toga, primjena kulture stanica omogućuje istraživanja molekularnih mehanizama toksičnosti, interakcija s receptorima i praćenje specifičnih unutarstaničnih promjena izazvanih djelovanjem toksikanta, dok in vivo studije ne omogućuju takve spoznaje. Većina alternativnih testova toksičnosti još je u postupku razvoja i validacije te se koriste samo kao predtestovi. U ovom radu preliminarno je ispitana mogućnost korištenja CHO-K1 stanične linije kao alternativnog test sustava u ispitivanju citotoksičnosti PCB-77 Trypan Blue metodom. Upotreba kulture CHO-K1 stanica omogućila je vrlo brzo, rutinski i relativno jeftino dobivanje osnovnih podataka o djelovanju odabranog kongenera PCB-a, čime se ovakav test sustav pokazao zadovoljavajućim.

Abstract (english)

Animal culture cells are used in industrial biotechnology and in many scientific disciplines to investigate the toxicity of xenobiotics. In the pharmaceutical industry, their application is very important in producing high-valuable preparations such as human and veterinary vaccines, hormones, neuroregulators etc. Inclusion of in vitro methods in toxicological invastigations is necessary to reduce the number of animals used in experiments. In addition, the use of cell culture allows investigation of molecular mechanisms of toxicity, interaction with receptors and monitoring of specific intracellular changes caused by the activity of toxicant, whereas in vivo studies do not provide such insights. The majority of alternative toxicity tests are still in the process of development and validation and are used only as preliminary tests. In this work it has been investigated the possibility of CHO-K1 cell line usage as an alternative test-system in studying the cytotoxicity of PCB-77 by Trypan Blue method. Utilisation of CHO-K1 culture enabled a very quick, relatively inexpensive and routinely obtaining of basical data on the activities of selected congener of PCBs, and this test-system has been proved as satisfactory.

• Keywords: kulture životinjskih stanica; in vitro testovi toksičnosti; CHO-K1 stanična linija; PCB-77; metoda Trypan Blue
• Keywords (english): animal culture cells; in vitro toxicity tests; CHO-K1 cell line; PCB-77; Trypan Blue method
• Language: croatian
• URN:NBN: urn:nbn:hr:159:393674
• Study programme: Title: Nutrition; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-06-07 11:59:12