Koflokulacija neflokulentnih stanica kvasca Saccharomyces cerevisiae s flokulentnim stanicama Saccharomyces uvarum

undergraduate thesis

• Title (english): Co-flocculation of non-flocculent yeast Saccharomyces cerevisiae cells by addition of flocculent yeast Saccharomyces uvarum cells
• Author: Hrvoje Žilić
• Mentor: Sunčica Beluhan (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology); Zagreb
• Defense date and country: 2010-09-09, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U radu je istraživana koflokulacija neflokulentnih stanica kvasca S. cerevisiae s flokulentnim stanicama pivskog kvasca S. uvarum. Koflokulacija je brzi proces (5 minuta), odvija se u širokom rasponu pH vrijednosti (3,0-8,0) i zahtijeva malu količinu kalcija (0,1 mM). Učinak miješanja je utjecao na agregaciju kvasca, no miješanje između 60 okr/min i 80 okr/min imalo je vrlo mali učinak na proces koflokulacije, dok je miješanjem uzoraka pri 100 okr/min postignut 91%-tni učinak koflokulacije, a pri 140 okr/min 94%-tna koflokulacija. Flokulentne stanice S. uvarum imaju sposobnost taloženja neflokulentnih stanica S. cerevisiae, što pokazuje prikladnost koflokulacije za separaciju različitih tipova neflokulentnih stanica.

Abstract (english)

Co-flocculation of non-flocculent yeast S. cerevisiae cells by addition of flocculent brewer’s yeast cells S. uvarum was investigated. Co-flocculation is a fast process (within 5 minutes), occurs in a broad pH range (3.0-8.0) and requires a small amount of calcium (0.1 mM). Agitation affects yeast aggregation; however, an agitation between 60 rpm and 80 rpm has only a little influence on the co-flocculation process, but agitation between 100 rpm and 140 rpm, co-flocculation reached 91 and 94%, respectively. Flocculent cells of S. uvarum had the ability to sediment non-flocculent cells of S. cerevisiae, which shows the suitability of the co-flocculation process for separation of different kinds of non-flocculent cells.

• Keywords: agregacija; flokulacija; koflokulacija; taloženje; kvasac
• Keywords (english): aggregation; flocculation; co-flocculation; sedimentation; yeast
• Language: croatian
• URN:NBN: urn:nbn:hr:159:958864
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-06-07 12:57:29