Učinak eutektičnih otapala na kulturu MCF-7 stanica

undergraduate thesis

• Title (english): The effect of deep eutectic solvents on MCF-7 cell line
• Author: Lukrecija Vlahović
• Mentor: Kristina Radošević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2016-06-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Eutektična otapala su smjese akceptora i donora vodikove veze, a imaju temperaturu tališta mnogo nižu od temperature tališta pojedinačnih komponenata smjese. Zbog svojih jedinstvenih fizikalno-kemijskih svojstava, niske cijene i široke mogućnosti primjene eutektična otapala su u fokusu interesa posljednjih godina. Industrijska upotreba eutektičnih otapala može se očekivati u bližoj budućnosti obzirom na veliku mogućnost primjene u području zelenih tehnologija, no prije upotrebe u velikom mjerilu, njihov utjecaj na okoliš te biorazgradivost i toksičnost mora se detaljnije istražiti.U ovom radu ispitan je utjecaj osam eutektičnih otapala na preživljenje humane stanične linije MCF-7 primjenom kolorimetrijske MTS metode. Na temelju dobivenih rezultata možemo zaključiti da ispitana eutektična otapala nemaju inhibitoran učinak na rast MCF-7 stanica te posjeduju nisku citotoksičnost.

Abstract (english)

Deep eutectic solvents are mixtures of hydrogen bond aceptors and hydrogen bond donors with a melting point much lower than that of either of its components. They have been in the focus of interest in the last years due to their unique properties, low cost and wide range od applications. Their industrial usage can be expected in the future based on numerous potencial DESs applications in the green technologies, therefore environmental impact and fate of DESs has to be investigated in more details. In this final work the impact of eight DESs on human cell line MCF-7 was tested by MTS method. Based on the results, we can conclude that these DESs have no inhibitory effect on MCF-7 cells and therefore possess low cytotoxicity.

• Keywords: eutektična otapala; MCF-7 stanice; ekotoksikologija
• Keywords (english): deep eutectic solvents; MCF-7 cells; ecotoxicology
• Language: croatian
• URN:NBN: urn:nbn:hr:159:346664
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 11:41:27