Učinak PCB 77 i PCB 153 na proliferaciju i stanični ciklus CHO (Chinese Hamster Ovary)-K1 stanica

master's thesis

• Title (english): Effect of PCB 77 and PCB 153 on proliferation and cell cycle of CHO (Chinese Hamster Ovary)-K1 cells
• Author: Martina Ivanjko
• Mentor: Ivana Kmetič (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2010-12-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

U ovom radu je ispitan učinak planarnog PCB 77 i neplanarnog PCB 153 na proliferaciju CHO (Chinese Hamster Ovary)-K1 stanica metodom Neutral Red tijekom 72 sata. Protočnom citometrijom određen je udio stanica u pojedinoj fazi staničnog ciklusa nakon obilježavanja stanične DNA propidij jodidom. Stanice su tretirane različitim koncentracijama PCB 77 i PCB 153 (10 – 100 μM). Pri svim ispitanim koncentracijama PCB 77, osim kod najniže (10 μM), primijećeno je statistički značajno smanjenje broja stanica u odnosu na kontrolu tijekom cjelokupnog perioda inkubacije. PCB 153 statistički je značajno inhibirao staničnu proliferaciju pri koncentracijama 75 μM i 100 μM nakon 24 i 48 sati, dok je nakon 72 sata inkubacije značajan inhibicijski učinak određen pri svim koncentracijama iznad 10 μM. Prema izračunatim IC vrijednostima PCB 77 je imao jače citotoksično djelovanje od PCB 153. PCB 77 i PCB 153 (50 μM) u odnosu na kontrolu nisu značajno promijenili stanični ciklus nakon 6 i 48 sati. Nešto izraženiji učinak određen je za PCB 77 u odnosu na PCB 153.

Abstract (english)

In this study the cytotoxic effects od planar PCB 77 and nonplanar PCB 153 were examined by monitoring CHO (Chinese Hamster Ovary )-K1 cell proliferation using Neutral Red method during 72 hours. After staining DNA with propidium iodid the amount of cells during particular phases of the cell cycle was determined using flow cytometry. Cells were treated with different concentrations of PCB 77 and PCB 153 (10 – 100 μM). Statistically significant decrease of cell proliferation was detected in all tested concentrations of PCB 77, apart 10 μM, during whole period of incubation. After 24 and 48 hours the highest tested concentrations of PCB 153 (75 and 100 μM) caused statistically significant decrease of cell proliferation, while after 72 hours such effect exerted all examined concentrations, apart 10 μM. PCB 77 was more cytotoxic according to calculated IC values. PCB 77 and PCB 153 did not significantly changed cell cycle after 6 and 48 hours comapared to control. However, PCB 77 affects the cell cycle in greater extent in comparison to PCB 153.

• Keywords: PCB 77; PCB 153; CHO-K1 stanična linija; citotoksičnost; stanični ciklus; Neutral Red metoda
• Keywords (english): PCB 77; PCB 153; CHO-K1 cell line; cytotoxicity; cell cycle; Neutral Red method
• Language: croatian
• URN:NBN: urn:nbn:hr:159:781599
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-29 10:57:04