Citotoksični, antioksidativni i prooksidativni karakter ekstrakta koprive Urtica dioica na humanim stanicama u kulturi

master's thesis

• Title (english): Cytotoxic, antioxidative and prooxidative nature of nettle extract Urtica dioica on human cell lines
• Author: Tonča Koludrović
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2010-12-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Kopriva (Urtica dioica) se tradicionalno primjenjuje u razne svrhe, a u posljednje vrijeme sve više je znanstvenih istraživanja koja potvrđuju njene pozitivne biološke učinke. Pripisuju joj se analgetsko, protuupalno, anti-proliferativno, antikancerogeno, antioksidativno i druga djelovanja. Cilj ovog rada bio je ispitati citotoksičnost koprive, učinak koji ima na razine slobodnih radikala (i na taj način utvrditi ima li antioksidativno ili prooksidativno djelovanje), te utjecaj na razine ukupnog glutationa u stanici, pri različitim uvjetima. Ispitivane su različite koncentracije ekstrakta koprive na stanicama raka grkljana, HEp2 i metastatskim stanicama debelog crijeva, SW 480. Prilikom ispitivanja učinka ekstrakta koprive na razinu slobodnih radikala i glutationa, praćen je i učinak na stanice u kojima je prethodno induciran oksidativni stres vodikovim peroksidom. Dobiveni rezultati pokazali su da ekstrakt koprive ima vrlo blagi citotoksični učinak na obje stanične linije, te da do naglog pada preživljenja dolazi tek pri najvećoj ispitivanoj koncentraciji. Povećanje netoksične koncentracije ekstrakta koprive dovodi do smanjenja razine slobodnih radikala, što ukazuje na njeno antioksidativno djelovanje, koje je ujedno i nešto veće kod stanica u kojima je prethodno izazvan oksidativni stres. Povećanjem koncentracije ekstrakta koprive dolazi do povećanja koncentracije ukupnog glutationa u HEp2 staničnoj liniji, dok je u staničnoj liniji SW 480 taj efekt izostao.

Abstract (english)

Nettle (Urtica dioica) has been traditionally used for various purposes, and lately more and more scientific research is confirming its positive biological effects. Analgetic, anti-inflammatory, anti-proliferative, anticancer, antioxidant and other activities have been attributed to it. The aim of this study was to examine the cytotoxicity of nettle, an effect that it has on the level of free radicals (and thus determine whether it has antioxidant or prooxidant effect), and the impact on total glutathione level in cells under different conditions. Different concentrations of nettle extract were tested on laryngeal cancer cells, HEp2 and metastatic colorectal cells, SW 480. When examining the effect of nettle extract on the level of free radicals and glutathione, the effect on the cells in which oxidative stress was induced (with hydrogen peroxide) was also determined. The results showed that the nettle extract has a very slight cytotoxic effect on both cell lines, and that a sharp decline in survival comes only at the highest concentration tested. Increasing nontoxic concentrations of nettle extract leads to decreased levels of free radicals, which points to its antioxidant activity, and it is also slightly higher in cells that were exposed to oxidative stress before the treatment. Total cellular glutathione level was increased in HEp2 cell lines in dose/response manner, while in SW 480 cell line this effect was not detected.

• Keywords: citotoksičnost; glutation; stanične linije; slobodni radikali; Urtica dioica
• Keywords (english): cell lines; cytotoxicity; free radicals; glutathione; Urtica dioica
• Language: croatian
• URN:NBN: urn:nbn:hr:159:330011
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-08-29 11:24:30