Lokalizacija proteina kontrolne točke diobenog vretena u mišjim spermatocitama

undergraduate thesis

• Title (english): Localization of spindle assembly checkpoint proteins in mouse spermatocytes
• Author: Vanessa Keser
• Mentor: Anamarija Štafa (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2016-06-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Tijekom mejoze, od velike je važnosti pravilna segregacija kromosoma, koja se ostvaruje zahvaljujući bipolarnom povezivanju niti diobenog vretena s kinetohorama kromosoma. Ukoliko bipolarno povezivanje nije ostvareno, kontrolna točka diobenog vretena (engl. Spindle Assembly Checkpoint, SAC) odgađa anafazu djelovanjem specifičnog kompleksa SAC, kojeg čine i proteini MAD1, MAD2, BUB1 i 3F3/2 fosfoepitop. Ako ova kontrolna točka nije funkcionalna, neostvarivanje bipolarne orijentacije diobenog vretena rezultira nepravilnom segregacijom kromosoma i promjenom broja kromosoma u stanicama kćeri. U ovome radu proteini MAD2 i 3F3/2 fosfoepitop, koji su dio kompleksa SAC, imunološki su obilježeni u svrhu određivanja njihove lokalizacije. Korišteni su transgeni Spo11β-only miševi, u čijim mejotičkim stanicama češće dolazi do nebipolarnog povezivanja kromosoma i mikrotubula nego kod miševa divljeg tipa. Utvrđeno je da proteini MAD2 i 3F3/2 fosfoepitop kolokaliziraju s proteinom SYCP3 u metafazi I.

Abstract (english)

During meiosis, appropriate segregation of chromosomes is of crucial importance, and it is achieved via bipolar orientation of kinetochore-microtubule attachment. Spindle assembly checkpoint (SAC), through a specific SAC complex consisting of MAD1, MAD2, BUB1 and 3F3/2 phosphoepitose and several other proteins, delays anaphase onset until all chromosomes are properly attached to the spindle. In case of malfunctioning SAC, cell will enter anaphase without proper kinetochore-microtubule orientation, and improper chromosomes segregation will occur, resulting in abnormal number of chromosomes in daughter cell. In this work proteins MAD2 and 3F3/2 phosphoepitope of the SAC complex were immunostained in order to determine their localization. Due to more frequent improper mitotic kinetochore-microtubule attachment, compared to the wild type, transgenic Spo11β-only mice were used. It was determined that both MAD2 and 3F3/2 phosphoepitope colocalize with SYCP3 in metaphase I.

• Keywords: kontrolna točka diobenog vretena; bipolarno povezivanje; Spo11β-only miševi
• Keywords (english): spindle assembly checkpoint; bipolar attachment; Spo11β-only mice
• Language: croatian
• URN:NBN: urn:nbn:hr:159:787761
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 13:16:19