Flavonoidni profil i biološka aktivnost vodenih ekstrakata samoniklih šafrana (Crocus L.) Hrvatske

master's thesis

• Title (english): Flavonoid profile and biological activity of Croatian wild saffron species (Crocus L.) water extract
• Author: Mirta Stipaničev
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2017-07-21, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Kultivirani šafran Crocus sativus L. od čijih se tučaka dobiva skupocjeni začin poznat je i po brojnim pozitivnim učincima na zdravlje. Međutim, postoje i druge nedovoljno istražene samonikle svojte roda Crocus L. koje su rasprostranjene uzduž Hrvatske, a sadrže bioaktivne tvari i mogle bi blagotvorno djelovati na zdravlje. Cilj ovog rada je analizirati flavonoidni sastav vodenih ekstrakata biljnih dijelova nekih samoniklih svojti roda Crocus L. te istražiti in vitro biološki utjecaj ekstrakata latica na CaCo-2 staničnoj liniji. U svrhu analize flavonoidnog sastava korištena je metoda tekućinske kromatografije visoke djelotvornosti, a biološki učinak je utvrđen mjerenjem citotoksičnosti i indukcije reaktivnih kisikovih radikala. Dijelovi cvijeta (prašnici, tučci, latice) imaju visok sadržaj glikozida kempferola i kvercetina, ali sastav i količina značajno variraju ovisno o svojti i dijelu cvijeta. Ekstrakti latica nisu pokazali citotoksično djelovanje pri analiziranim koncentracijama, pritom su neki od njih značajno snizili indukciju radikala. Flavonoidnim sastavom i biološkim djelovanjem istaknula se ljubičasta forma svojte C. vernus ssp. vernus.

Abstract (english)

Cultivated saffron (Crocus sativus L.) from whose stigmas expensive spice saffron is made, is also known for its many benefical health effects. However, there are other scarcely researched wild Crocus L. taxa that grow in Croatia, contain bioactive compounds and could have positive effects on health. The aim of this thesis was to analyze flavonoid composition in water extracts of some wild Crocus L. species taxa and to investigate the biological activity of petal extracts on CaCo-2 cell line. To analyze the flavonoid profile, high performance liquid chromatography was used and biological activity was determined by measuring cytotoxicity and induction of reactive oxygen species. Flower parts (stamens, stigmas, petals) are rich in kaempferol and quercetin glycosides, but the composition and the quantitiy of these compounds vary depending on the specie and the flower part. Petal extracts haven't shown cytotoxic activity in analyzed concentrations though some of the extracts decreased the induction of radicals. Considering its flavonoid composition and in vitro biological acitivity, purple form of C. vernus ssp. vernus is shown to be the most prominent among sampled taxa.

• Keywords: citotoksičnost; Crocus L.; flavonoidi; HPLC; reaktivne kisikove vrste
• Keywords (english): cytotoxicity; Crocus L.; flavonoids; HPLC; reactive oxygen species
• Language: croatian
• URN:NBN: urn:nbn:hr:159:955951
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-07 11:19:10