Utjecaj prebiotika Nutriose®FB06 na sastav crijevne mikrobiote u uvjetima in vitro

master's thesis

• Title (english): Influence of prebiotic Nutriose®FB06 on the composition of gut microbiota in an in vitro system
• Author: Tena Čadež
• Mentor: Jurica Žučko (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Section for Bioinformatics); Zagreb
• Defense date and country: 2017-09-22, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Mikrobiota kao zajednica mikroorganizama koji obitavaju na i u ljudskom tijelu je postala područje istraživanja zbog svog značajnog utjecaja na zdravlje i dobrobit ljudi. Razvojem naprednih tehnika sekvencioniranja omogućeno je određivanje sastava crijevne mikrobiote te razvojem in vitro sustava praćenje njenih promjena pod utjecajem određenim čimbenika. U ovom radu se istraživao učinak prebiotika Nutriose®FB06 na sastav crijevne mikrobiote čovjeka. Iz tog razloga, korišten je in vitro sustav za simulaciju ljudskog debelog crijeva, a promjene mikrobiote su istraživane sekvencioniranjem 16S rRNA gena. Dobiveni rezultati ukazuju da dodavanje prebiotka potiče održavanje bioraznolikost crijevne mikrobiote u bioreaktoru. Također je uočeno da povećane koncentracije prebioika utječu na veću zastupljenost bakterijskiog roda Bifidobacterium te je u budućim istraživanjima potrebno ustanoviti optimalnu koncentraciju prebiotika koja potiće održavanje većeg broja poželjnih bakterijskih rodova.

Abstract (english)

Microbiota, as a community of microorganisms living on and within the human body, has become an area of research because of its significant impact on the health and well-being of humans. With development of advanced sequencing techniques, it was possible to determine the composition of the gut microbiota and by developing in vitro systems it was possible to track its changes induced by certain factors. The aim of this thesis was to determine the effect of Nutriose® FB06 prebiotics on the intestinal microbiotic composition in humans. For this reason, an in vitro human colon simulation system was used, and changes in microbiota were investigated by sequencing the 16S rRNA gene. The obtained results indicate that adding prebiotics encourages the maintenance of intestinal microbial biodiversity in the bioreactor. It has also been found that increased prebiotic concentrations have a greater influence on bacterial genus Bifidobacterium, and in future studies it is necessary to establish optimal concentration of prebiotics that will stimulate the growth of a higher number of desirable bacterial genera.

• Keywords: crijevna mikrobiota; in vitro sustav; prebiotik; 16S rRNA gen
• Keywords (english): gut microbiota; in vitro systems; prebiotics; 16S rRNA gene
• Language: croatian
• URN:NBN: urn:nbn:hr:159:448423
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-11-13 14:44:20