Primjena proteomike i drugih molekularnih metoda u definiranju funkcionalnih svojstava probiotičkih bakterija

doctoral thesis

• Title (english): Application of proteomics and other molecular methods in characterisation of functionality of the probiotic bacteria
• Author: Jasna Beganović; MBZ: 267973
• Mentor: Jagoda Šušković (mentor); MBZ: 40116
• Committee member: Vladimir Mrša (predsjednik povjerenstva); MBZ: 39525
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2008-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 60 - Biotechnology. Genetic engineering. Products of cloning

Abstract

Cilj ovog rada je definiranje funkcionalnih svojstava dviju bakterija mliječne kiseline na molekularnoj razini: bakterije Lactococcus lactis koja je modelni mikroorganizam za proučavanje fiziologije BMK i probiotičke bakterije Lactobacillus acidophilus M92. U tu svrhu primjenjene su komplementarne molekularne metode, u prvom redu proteomičke. Razvijeni postupci su omogućili identifikaciju 490 proteina netopljivog proteoma koji je nadopunjuje citoplazmatski proteom bakterije L. lactis. Zbog mogućnost primjene L. lactis kao živog prijenosnika vakcina ili heterolognih proteina, analiziran je i netopljivi proteom ove bakterije nakon prolaska kroz gastrointestinalni sustav miša. Statistička analiza rezultata omogućila je usporedbu proteoma bakterije L. lactis iz dvaju različitih mikrookoliša, te identifikaciju proteinskih markera adaptacije na stresne uvjete gastrointestinalnog trakta. Proteomički pristup primjenjen za L. lactis, također se primjenio za analizu proteoma probiotičkog soja L. acidophilus M92 s posebnim osvrtom na SlpA površinske proteine koji imaju nekoliko različitih primjena, uključujući njihovu uporabu kao nositelja vakcina.

Abstract (english)

The aim of this research was a characterisation of functional properties of two lactic acid bacteria (LAB) on a molecular level: Lactococcus lactis, which is a model bacterium for studying LAB physiology and probiotic strain Lactobacillus acidophilus M92. Therefore, complementary molecular methods were applied and prior proteomics. Developed approaches provided identification of 490 different proteins of an insoluble L. lactis proteome. The analysis of L. lactis insoluble proteome after the passage of this bacterium through the gastrointestinal tract (GIT) of mice was undertaken as there is a potential of L. lactis use as live oral vector for vaccine and heterologous proteins. Statistical analysis enabled comparison L. lactis proteomes from two very different environments and identification of protein biomarkers of adaptation of this bacterium to GIT. The proteomic approach developed on L. lactis, was applied for the proteome analysis of probiotic strain L. acidophilus M92 with a special focus on SlpA surface proteins which already have a few applications including their use as an antigen delivery vehicles.

• Keywords: bakterijska adaptacija; Lactobacillus acidophilus M92; Lactococcus lactis IL1403; probiotici; proteini stanične ovojnice; proteomika; spektrometrija masa; vaccine
• Keywords (english): bacterial adaptation; Lactobacillus acidophilus M92; Lactococcus lactis IL1403; probiotics; cell envelope proteins; proteomics; mass spectrometry; vaccine
• Language: croatian
• URN:NBN: urn:nbn:hr:159:210428
• Promotion: 2009-09-13
• Study programme: Title: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-14 08:56:57