Primjena kulture životinjskih stanica na studiju citotoksičkih učinaka pesticida

doctoral thesis

• Title (english): Application of animal cell cultures in the study of cytotoxic effects of pesticides
• Author: Ivana Kmetič; MBZ: 249940
• Mentor: Jasna Kniewald (mentor); MBZ: 21481
• Committee member: Zlatko Kniewald (predsjednik povjerenstva); MBZ: 71903
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2008-07-10, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 631/632 - Agriculture. Plant diseases. Plant protection

Abstract

Toksični učinci atrazina i četiri organoklorirana pesticida ispitivani su na staničnoj razini. Korištene su Chinese Hamster Ovary (CHO-K1) i Baby Hamster Kidney (BHK- 21 [C-13]) stanične linije u utvrđivanju stupnja in vitro citotoksičnosti pesticida i praćenju morfoloških promjena pri odumiranju stanica. Primijenjene su Trypan Blue, Neutral Red, Kenacid Blue i MTT metode te su određene IC50 vrijednosti tijekom 72 sata izloženosti pesticidima.

Abstract (english)

The toxic effects of atrazine and four organochlorine pesticides were examined at the cellular level. Chinese Hamster Ovary (CHO-K1) and Baby Hamster Kidney (BHK-21 [C-13]) cell lines were used to evaluate the degree of pesticides' cytotoxicity in vitro and the morphological changes were followed during the cell death. Trypan Blue exclusion method, Neutral Red, Kenacid Blue and MTT assays were applied, and IC50 values were determined with each assay up to 72 h of exposure.

• Keywords: CHO-K1 stanice; BHK-21 [C-13] stanice; citotoksičnost; atrazin; lindan; heptaklor; 2; 4-D; aldrin; IC50 vrijednosti; apoptoza; nekroza
• Keywords (english): CHO-K1 cells; BHK-21 [C-13] cells; cytotoxicity; atrazine; lindane; heptachlor; 2; 4-D; aldrin; IC50 values; apoptosis; necrosis
• Language: croatian
• URN:NBN: urn:nbn:hr:159:837572
• Promotion: 2009-09-13
• Study programme: Title: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-14 09:56:32