Karakterizacija rekombinantnog humanog faktora stimulacije rasta granulocita i utvrivanje korelacije kromatografskih tehnika s postupkom određivanja biološke aktivnosti

doctoral thesis

• Title (english): Characterization of recombinant human granulocyte colony stimulating factor and determination of correlation between chromatographic techniques and biological assay
• Author: Ana Škrlin; MBZ: 248160
• Mentor: Vladimir Mrša (mentor); MBZ: 39525
• Committee member: Božidar Šantek (predsjednik povjerenstva); MBZ: 184956
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2011-05-05, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

Filgrastim (rekombinantni humani faktor stimulacije rasta granulocita) je biotehnološki proizvedena aktivna supstancija, korištena u liječenju neutropenie. U ovom radu provedena je ekstenzivna analitička karakterizacija filgrastima, te izolacija i identifikacija filgrastimu srodnih onečišćenja. Utvrđena je značajna korelacija između udjela filgrastimu srodnih onečišćenja i vrijednosti biološke aktivnosti u uzorcima filgrastima. Predložen je regresijski model pogodan za procjenu biološke aktivnosti uzoraka filgrastima na temelju rezultata dobivenih reverzno-faznom kromatografijom.

Abstract (english)

Filgrastim (recombinant human granulocyte colony stimulating factor) is biotechnology derived product, used for the treatment of neutropenia. In this research extensive analytical characterization of filgrastim molecule was performed. Relevant filgrastim related impurities were purified to homogeneity and characterized by liquid chromatography and mass spectrometry. Significant correlation between chromatography results and biological activity for filgrastim samples was confirmed, and regression model suitable for the estimation of biological activity was established.

• Keywords: biološka aktivnost; filgrastim; korelacija; kromatografija; regresijski model; onečišćenje
• Keywords (english): biological assay; chromatography; correlation; filgrastim; impurity; regression model
• Language: croatian
• URN:NBN: urn:nbn:hr:159:981551
• Promotion: 2011-11-06
• Study programme: Title: Biotechnology & Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor znanosti/doktorica znanosti (doktor znanosti/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-15 11:16:11