Priprava, primjena u biotransformacijama i citotoksičnost odabranih imidazolijevih ionskih tekućina

doctoral thesis

• Title (english): Synthesis, application in biotransformations and cytotoxicity of selected imidazolium-based ionic liquids
• Author: Marina Cvjetko Bubalo
• Mentor: Jasna Vorkapić-Furač (mentor)
• Committee member: Višnja Gaurina-Srček (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2012-11-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology
• Universal decimal classification (UDC): 66 - Chemical technology. Chemical and related industries

Abstract

Pripravljene nove imidazolijeve ionske tekućine ispitane su kao otapala u lipazom kataliziranoj sintezi izoamil-acetata te je uspostavljena visokoproduktivna kontinuirana sinteza izoamil-acetata u mikroreaktoru s nasutim slojem imobilizirane lipaze (61 mmol L−1 min−1). Primjenom nekonvencionalnih metoda u pripravi ionskih tekućina moguće je znatno skratiti vrijeme reakcije i smanjiti potrošnju energije. Ispitivanjem citotoksičnosti na CCO staničnoj liniji utvrđeno je da imidazolijeve ionske tekućine uglavnom ispoljavaju umjerenu toksičnost (EC50 = 0,1 – 10 mmol L-1), induciranu oštećenjem stanične membrane i posljedičnom nekrozom.

Abstract (english)

Novel imidazolium ionic liquids were prepared and tested as solvents for enzyme-catalyzed isoamyl acetate synthesis and highly productive continuous esterification in an immobilized lipase packed-bed microreactor was developed (61 mmol L−1 min−1). The use of unconventional methods for ionic liquids’ synthesis contributes to the reduction of preparation time as well as energy consumption. Results of in vitro cytotoxicity assays indicate moderate toxicity of imidazolium ionic liquids (EC50 = 0,1 – 10 mmol L-1) through the mechanism of membrane disruption followed by necrosis.

• Keywords: biotransformacije; CCO stanice; citotoksičnost; ionske tekućine; izoamil-acetat; kvaternizacija; lipaza; mikroreaktori; mikrovalno zračenje; ultrazvuk
• Keywords (english): biotransformation; CCO cells; cytotoxicity; ionic liquids; isoamyl acetate; lipase; microreactors; microvawe irridiation; quaternization; ultrasound
• Language: croatian
• URN:NBN: urn:nbn:hr:159:056224
• Promotion: 2013-06-30
• Study programme: Title: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-16 10:43:56