Spektrofotometrijsko određivanje smjese prehrambenih bojila uz kemometričku separaciju višeanalitnog signala

professional thesis

• Title (english): Spectrophotometric determination of food dye mixture with chemometric separation of multianalyte signal
• Author: Iva Grabarić Andonovski
• Mentor: Nada Vahčić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; Zagreb
• Defense date and country: 2015-05-18, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Boja je prvo senzorsko svojstvo prema kojem se ocjenjuje kakvoća hrane. Stoga se hrani dodaju sintetska prehrambena bojila, da bi se nadoknadio gubitak prirodne boje uslijed prerade sirovina. Analitička kontrola prisutnosti sintetskih bojila u hrani vrlo je važna zbog brojnih rizika koje bojila predstavljaju za ljudsko zdravlje. Koncentracija prehrambenih bojila može se jednostavno i brzo odrediti spektrofotometrijom, no kod određivanja smjese bojila u prehrambenim proizvodima često dolazi do preklapanja signala komponenata prisutnih u smjesi. Stoga je u radu istraženo može li se primjenom rezolucijske metode omjera signala odrediti koncentracija triju prehrambenih bojila prisutnih u uzorku gaziranog bezalkoholnog pića. Uporabom sintetskih smjesa prehrambenih bojila određeni su parametri metode, čime se znatno skratio eksperimentalni postupak. Analizom realnog uzorka potvrđeno je da se metoda može primijeniti za određivanje koncentracije prehrambenih bojila u smjesi, s pogreškom prihvatljivom na mikromolarnoj razini. Koncentracije bojila u uzorku bezalkoholnog gaziranog pića bile su unutar zakonski propisanih granica.

Abstract (english)

Colour is one of the major food properties. During food processing part of its natural colour is lost, hence synthetic food dyes are added to the food. Due to the possible negative impact of synthetic dyes on human health, their presence should be monitored. Concentration of food dyes can easily be determined spectrophotometrically. However, the main obstacle for determining food dyes in a mixture is the overlapping of their signals. In this work, a signal ratio method was applied to resolve the signals of three different food dyes in a soft drink. To shorten the procedure, method parameters were optimised using synthetic food dye mixtures. It was confirmed by real sample analysis that the method can be successfully applied for determining concentration of food dyes in a mixture, with an acceptable error level at micromolar scale. Concentrations of food dyes in the soft drink were within regulatory limits.

• Keywords: spektrofotometrijska analiza; prehrambena bojila; gazirano bezalkoholno piće; razdvajanje signala smjese; metoda omjera signala
• Keywords (english): spectophotometric analysis; food dyes; soft drink; signal resolution; signal ratio method
• Language: croatian
• URN:NBN: urn:nbn:hr:159:692057
• Study programme: Title: Food Management; Study programme type: university; Study level: postgraduate specialist; Academic / professional title: sveučilišni/a specijalist/specijalistica upravljanja hranom (sveučilišni/a specijalist/specijalistica upravljanja hranom)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2018-05-18 09:20:18