Utjecaj polifenolna iz ekstrakta cvijeta trnine na dijabetes tipa 2 u C57BL/6 miša

undergraduate thesis

• Title (english): Influence of Prunus spinosa L. flower extract polyphenols on type-2 diabetes mellitus in C57BL/6 mouse
• Author: Tajana Frančić
• Mentor: Irena Landeka Jurčević (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Chemistry and Biochemistry); Zagreb
• Defense date and country: 2018-07-02, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Brojni dokazi sugeriraju da dijeta bogata polifenolnim spojevima ima mogućnost zaštite od dijabetesa. Ekstrak cvijeta Prunus spinosa L. je bio promatran zbog svog utjecaja na lipidnu peroksidaciju i antioksidativni status kod miševa kod kojih je dijabetes izazvan pomoću alloxana. Promatrani biomarkeri oksidativnog stresa u organima su bili malonilaldehid (MDA) i reducirani glutation (GSH). Primjena ekstrakta cvijeta P. spinosa L. u dozi od 100 mg/kg tjelesne mase na C57BL/6 dijabetičnim miševima tijekom 10 dana je uzrokovala značajnu redukciju povišenih MDA vrijednosti, istovremeno povećavajući aktivnost antioksidativnog enzima (GSH) na dijabetičnim miševima. Promatrano značajno povećanje razine MDA u tkivima jetre i bubrega, kod dijabetičnih miševa, sugerira da dijabetični miševi imaju povećanu koncentraciju slobodnih radikala u usporedbi s normalnim, zdravim miševima. Smanjena aktivnost GSH u jetri i bubrezima tijekom dijabetesa je vjerojatno zbog inaktivacija ili inhibicije enzima i to zbog povećanje proizvodnje aloksan povezanih reaktivnih kisikovih vrsta (ROS). Rezultati ukazuju da ekstrakt cvijeta Prunus spinosa L. može reducirati slobodne radikale povezane s oksidativnim stresom u eksperimentalno izazvanom diabetes mellitus.

Abstract (english)

Significant evidence suggests that polyphenol-rich diets have the ability to protect against diabetes. Extract flower Prunus spinosa L. was investigated for its effects on lipid peroxidation and antioxidant status in alloxan-induced diabetic mice. The investigated biomarkers of oxidative stress in the organs were malondialdehyde (MDA) and reduced glutathione (GSH). Administration of extract of P. spinosa L. flower at a dose of 100 mg/kg body weight to C57BL/6 diabetic mice for 10 days caused significant reduction in the elevated MDA level, while increasing the activity of the antioxidant enzyme (GSH) in diabetic mice. The observed significant elevation in MDA level in liver and kidney tissues of alloxaninduced diabetic mice suggests an intensified free radical generation in diabetic mice compared to the normal mice. The lower activities of GSH observed in liver and kidney during diabetes may be due to the inactivation or inhibition of the enzymes by the increased production of alloxan-generated reactive oxygen species (ROS). Results indicate that the extract flower Prunus spinosa L. can reduce free radical mediated oxidative stress in experimental diabetes mellitus.

• Keywords: trnina; dijabetes tipa 2; malondialdehid (MDA); glutation (GSH)
• Keywords (english): trnina; type-2 diabetes mellitus; malondialdehyde (MDA); glutathione (GSH)
• Language: croatian
• URN:NBN: urn:nbn:hr:159:865276
• Study programme: Title: Nutrition; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-18 10:21:05