Utjecaj ljekovite kadulje (Salvia officinalis L.) na patogene mikroorganizme iz skupine Oomycetes

undergraduate thesis

• Title (english): The effect of common sage (Salvia officinalis L.) on pathogenic Oomycetes
• Author: Marija Škof
• Mentor: Ana Bielen (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2018-06-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Patogeni iz skupine Oomycetes napadaju poljoprivredne kulture i uništavaju usjeve, ali i vodene organizme značajne za akvakulturu. Njihovo se suzbijanje vrši uz pomoć pesticida, fungicida i sličnih kemikalija štetnih za čovjeka i okoliš. Cilj ovog rada bio je utvrditi mogu li ekstrakti listova kadulje (Salvia officinalis) inhibirati rast micelija oomiceta Aphanomyces astaci (patogen riječnih rakova) te Phytophtora plurivora i Phytophtora cactorum (biljni patogeni). Korišteni su pripravci kadulje ekstrahirani u etanolu, metanolu te istim otapalima razrijeđenima s vodom 1:1. Rast vrsta iz roda Phytophthora nije bio značajno inhibiran, vjerojatno jer se uslijed duge koevolucije s biljnim domaćinima kod ovih vrsta razvila otpornost na biljne metabolite. Međutim, vrsta A. astaci bila je značajno inhibirana ekstraktima kadulje (rast micelija smanjen za 65 do 70% u odnosu na kontrolu). Rezultati ukazuju na mogućnost primjene ekstrakata kadulje u kontroli patogenih oomiceta u slatkovodnoj akvakulturi.

Abstract (english)

Oomycete pathogens negatively affect agriculture and destroy crops, as well as organisms important in aquaculture. They are controlled by pesticides, fungicides and other chemicals that are harmful for humans and the environment. The object of this study was to determine the effect of sage leaf extracts on the mycelium growth of Aphanomyces astaci (freshwater crayfish pathogen), Phytophthora plurivora and Phytophthora cactorum (plant pathogens). Sage preparations which were used have been extracted in methanol, ethanol and the same solvents diluted with water in 1:1 ratio. The growth of Phytophthora species was not significantly inhibited, probably because of the long co-evolution with their hosts which could have made them resistant to host metabolites. On the other hand, A. astaci was significantly inhibited with sage extracts: growth was reduced from 65 to 70% in comparison to the control. Results indicate that sage extracts could be used for the control of oomycete pathogens in freshwater aquaculture.

• Keywords: Aphanomyces; Phytophthora; Oomycetes; kadulja
• Keywords (english): Aphanomyces; Phytophthora; Oomycetes; sage
• Language: croatian
• URN:NBN: urn:nbn:hr:159:174451
• Study programme: Title: Biotechnology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-19 09:10:24