Antioksidativna aktivnost odabranih sojeva bakterija mliječne kiseline

undergraduate thesis

• Title (english): Antioxidant activity of selected strains of lactic acid bacteria
• Author: Jelena Novak
• Mentor: Ksenija Markov (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for General Microbiology and Food Microbiology); Zagreb
• Defense date and country: 2018-09-20, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Antioksidativna aktivnost jedan je od važnih parametara prilikom karakterizacije bakterija mliječne kiseline (BMK) kao probiotika. Bakterijske stanice mogu producirati određene molekule koje im služe kao obrana od oksidativnog stresa. Primjenom takvih bakterija u prehrani ljudi, posebice BMK, pozitivni učinci prenose se na korisnika i smanjuju oksidacijska oštećenja u gastrointestinalnom sustavu. Cilj ovog rada bio je ispitati antioksidativni kapacitet odabranih sojeva BMK koji su u postupku probiotičke karakterizacije. Ispitana su tri glavna parametra antioksidativnog kapaciteta: aktivnost enzima superoksid dismutaze, mogućnost uklanjanja DPPH radikala i mjerenje koncentracije ukupnog glutationa. Na osnovu navedenih parametara odabrani sojevi su rangirani prema antioksidativnom potencijalu.

Abstract (english)

Antioxidant activity is important parameter when characterizing lactic acid bacteria (LAB) as potential probiotics. Bacterial cells can produce certain molecules that serve them as a defense against oxidative stress. By applying such bacteria to the diet of humans, especially LAB, positive effects are transferred to the user and reduce oxidative damage in the gastrointestinal system. The aim of this study was to examine the antioxidant capacity of the selected strains of LAB that are in the process of probiotic characterization. Three main parameters of antioxidant capacity were investigated: the enzyme activity of superoxide dismutase, DPPH free radical scavenging and concentration of total intracelular glutathione. Based on the mentioned parameters, the selected strains are ranked according to their antioxidant potential.

• Keywords: antioksidant; bakterije mliječne kiseline; DPPH; glutation; superoksid dismutaza
• Keywords (english): antioxidant; DPPH; gluthatione; lactic acid bacteria; superoxide dismutase
• Language: croatian
• URN:NBN: urn:nbn:hr:159:955599
• Study programme: Title: Food Technology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-09-25 12:01:10