Izolacija frakcija fenolnih spojeva iz sjemenki komorača primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku

undergraduate thesis

• Title (english): Isolation of polyphenols fractions from fennel seeds using pressurized liquid extraction
• Author: Ana Marija Medved
• Mentor: Maja Repajić (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Engineering); (Laboratory for Technology of Fruits and Vegetables Preservation and Processing); Zagreb
• Defense date and country: 2019-06-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Komorač je bogat izvor različitih skupina fenolnih spojeva, a za povećanje učinkovitosti njihove ekstrakcije razvijaju se nove tehnike. Stoga je cilj ovog istraživanja bio optimirati uvjete izolacije hidroksicimetnih kiselina, flavonola i flavan-3-ola iz odmašćenih sjemenki gorkog komorača sukcesivnim iscrpljivanjem 30 %-tnom otopinom acetona i 30 %-tnom otopinom metanola primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku (PLE) uz varirane parametre: temperatura (75 i 100 oC) te broj ciklusa (1, 2 i 3 ciklusa). Optimalni PLE uvjeti pri kojima su dobiveni najveći prinosi hidroksicimetnih kiselina bili su ekstrakcija s 30 %-tnim acetonom pri temperaturi od 100 oC i 3 ciklusa te ekstrakcija s 30 %-tnim metanolom pri 100 oC i 2 ciklusa. Najveći prinosi flavonola dobiveni su ekstrakcijom s 30 %-tnim acetonom te 30 %-tnim metanolom pri 100 oC i 3 ciklusa, dok je najveći prinos flavan-3-ola dobiven ekstrakcijom s 30 %-tnim acetonom pri 100 oC te 2 ciklusa.

Abstract (english)

Fennel is a rich source of different groups of phenolic compounds and new techniques are being developed in order to increase the efficiency of their extraction. Therefore, the aim of this study was to optimize the conditions for the isolation of hydroxycinnamic acids, flavonols and flavan-3-ols from defatted bitter fennel seeds by successive exhaustion with 30 % acetone solution and 30 % methanol solution using pressurized liquid extraction (PLE) with varied parameters: temperature (75 and 100 oC) and number of cycles (1, 2 and 3 cycles). The optimal PLE conditions for obtaining the highest yields of hydroxycinnamic acids were extraction with 30 % acetone at 100 oC and 3 cycles and extraction with 30 % methanol at 100 oC and 2 cycles. The highest flavonol yields were obtained by extraction with 30 % acetone and 30 % methanol at 100 oC and 3 cycles, while the highest yield of flavan-3-ols was obtained by extraction with 30% acetone at 100 oC and 2 cycles.

• Keywords: flavan-3-oli; flavonoli; hidroksicimetne kiseline; komorač; PLE
• Keywords (english): flavan-3-ols; flavonols; hydroxycinnamic acids; fennel; PLE
• Language: croatian
• URN:NBN: urn:nbn:hr:159:344927
• Study programme: Title: Nutrition; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-06-10 12:13:47