Određivanje alergena kikirikija u uzorcima keksa i čajnih peciva imunoenzimskom metodom

master's thesis

• Title (english): Determination of peanut allergens in samples of cookies and tea biscuits by immunoenzymatic method
• Author: Martina Tolvajčić
• Mentor: Ksenija Marković (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Food Quality Control); (Laboratory for Food Quality Control); Zagreb
• Defense date and country: 2018-07-19, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Tijekom ovog istraživanja, imunoenzimskom ELISA (engl. Enzyme-Linked Immunosorbent Assay) metodom analizirani su udjeli potencijalnih alergena kikirikija u 28 uzoraka keksa i čajnih peciva iz trgovačkih lanaca, pekarnica i slastičarnica te su prikupljeni stavovi i mišljenja potrošača o kikirikiju i proizvodima od kikirikija kao tvarima ili proizvodima koji uzrokuju alergije. U svih deset analiziranih uzoraka keksa i čajnih peciva iz trgovačkih lanaca detektirani su alergeni kikirikija, a prosječni udio iznosio je 0,37 mgkg-1. Alergeni kikirikija detektirani su u devet od deset uzoraka keksa i čajnih peciva iz pekarnica, a njihov udio iznosio je prosječno 0,38 mgkg-1. Prosječni udio alergena kikirikija, detektiranih u osam analiziranih uzoraka keksa i čajnih peciva iz slastičarnica, iznosio je 0,28 mgkg-1. Rezultati ispitivanja stavova i mišljenja potrošača pokazali su da je 84,8 % ispitanika upoznato sa pojmom alergije te također 39,2 % ispitanika smatra kako se kikiriki ubraja u orašaste plodove. Od dvadeset i osam analiziranih uzoraka keksa i čajnih peciva, ELISA metodom detektirani su alergeni kikirikija u dvadeset sedam uzoraka pri čemu su samo dva uzorka iz trgovačkog lanca sadržavala navod o mogućoj prisutnosti alergena kikirikija.

Abstract (english)

During this study, the content of potential peanut allergens in 28 samples of cookies and tea biscuits from supermarkets, bakeries and pastry shops was analysed by ELISA (Enzyme-Linked Immunosorbent Assay) method, and also the consumers opinion on peanut and peanut products as substances or products that cause allergies was examined. The presence of peanut allergens was detected in ten analysed samples of cookies and tea biscuits from supermarkets, with an average value of 0,37 mgkg-1. Peanut allergens were detected in nine of ten samples of cookies and tea biscuits from bakeries, with an average value of 0,38 mgkg-1. The average content of peanut allergens, detected in eight analysed samples of cookies and tea biscuits from pastry shops, was 0,28 mgkg-1. The results of consumers attitudes and opinions showed that 84,8 % of respondents are familiar with the term of allergy and also 39,2 % of respondents consider peanuts as tree nuts. Among twenty eight analysed samples of cookies and tea biscuits by ELISA method, peanut allergens were detected in twenty seven samples of which only two from supermarkets had advisory label related to the presence of peanut allergens.

• Keywords: alergeni; kikiriki; ELISA; informacija o hrani
• Keywords (english): allergens; peanut; ELISA; food information
• Language: croatian
• URN:NBN: urn:nbn:hr:159:045717
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-02 13:16:58