Utjecaj arbutina na inhibiciju bakterijske adhezije i promjenu antioksidacijskog potencijala

master's thesis

• Title (english): Arbutin effect on inhibition of bacterial adhesion and antioxidative potential change
• Author: Dominika Puljić
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2019-07-16, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Food Technology

Abstract

Arbutin, biološki aktivan spojprisutan u mnogim biljkama, ima široku primjenu zbog svojih antibakterijskih, antioksidacijskih, protuupalnih i antitumorskih svojstava, ali svoju najveću primjenu ima u liječenju infekcija mokraćnog sustava kao sastavni dio ekstrakta biljnih pripravaka.U ovom se radu proučavao i uspoređivao citotoksični učinak arbutina na bakterijama Escherichia coli i Lactobacillus plantarum te utjecaj arbutina na njihovu adheziju za humane epitelne stanice gastrointestinalnog trakta u in vitro sustavu. Prethodno adheziji, napravljeni su testovi citotoksičnosti Neutral red metodom i testovi potencijalnog antioksidacijskog djelovanja arbutina DCHF-DA metodom na tumorskim epitelnim stanicama jezika (CAL 27), kolorektalnog adenokarcinoma (Caco-2) i jetre (HepG2). Dobiveni podaci ukazuju na veći antimikrobni karakter arbutina na Gram-pozitivnu bakteriju (L.plantarum) u odnosu na Gram-negativnu bakteriju (E.coli) te na svojstvo arbutina da uzrokuje promjenu adhezije bakterijskih stanica za epitelne stanice probavnog sustava. Antioksidacijska aktivnost arbutina te stimulacija proliferacije humanih stanica ovisi o koncentraciji arbutina i vrsti tretiranih stanica.

Abstract (english)

Arbutin, a biologically active compound widely distributed among plants, has wide application for its antibacterial, antioxidative, anti-inflammatory and anticancer properties, but its greatest use is in the treatment of urinary tract infections as an integral part of the herbal extracts. In this thesis the cytotoxicity effect of arbutin on bacteria Escherichia coli and Lactobacillus plantarum was studied. Furthermore, the influence of arbutin on adhesion of mentioned bacteria to human epithelial gastrointestinal tract cells in the in vitro system was studied. Before the adhesion tests, cytotoxicity tests using Neutral red method were carried out together with the tests of potential antioxidant activity of the arbutin using DCHF-DA method on tumor epithelial cells of tongue (CAL 27), colorectal adenocarcinoma (Caco-2) and of liver (Hep G2). The obtained data indicate a higher antimicrobial character of the arbutin on Gram-positive bacteria (L.plantarum) compared to the Gram-negative bacterium (E.coli) and also its ability to cause changes in the adhesion of bacterial cells to the epithelial cells of the digestive system. Antioxidant activity of arbutin and stimulation of human cell proliferation depend on the concentration of arbutin and the type of treated cells.

• Keywords: arbutin; antioksidacijski potencijal; adhezija bakterija; citotoksičnost; humane stanične linije
• Keywords (english): arbutin; antioxidant activity; toxicity; bacterial adhesion; human cell lines
• Language: croatian
• URN:NBN: urn:nbn:hr:159:284435
• Study programme: Title: Food Safety Management; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane (magistar/magistra inženjer/inženjerka upravljanja sigurnošću hrane)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2019-12-04 13:16:26