Genotoksični učinak Δ⁹ -tetrahidrokanabinola (THC) na ljudske tumorske stanice jezika, jetre i crijeva

master's thesis

• Title (english): Genotoxic effect of Δ⁹- tetrahydrocannabinol (THC) on human oral, liver and colon tumor cells
• Author: Sara Stoček
• Mentor: Ksenija Durgo (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2019-10-30, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Kolorektalni karcinom najčešći je uzrok smrti nakon bolesti srca i krvožilnog sustava, a podjednako zahvaća muškarce i žene. Liječenje kolorektalnog karcinoma uključuje kirurško liječenje te primjenu kemoterapije i zračenja. THC je glavna aktivna komponenta biljke Cannabis sativa, a u zadnje se vrijeme sve više koristi kao suportivna terapija kemoterapiji. Cilj ovog rada je istražiti citotoksičan, prooksidativan i genotoksičan učinak THC-a. Kao test sustavi korištene su humane stanične linije epitelnih stanica karcinoma jezika (Cal 27), hepatocelularnog karcinoma jetre (Hep G2) i adenokarcinoma epitela debelog crijeva (Caco-2). Stanice su bile izložene djelovanju THC-a tijekom 2 – 48 sati, u rasponu koncentracija 0,33 μg mL-1 do 800 μg mL-1. Dokazano je da THC djeluje citotoksično ovisno o duljini tretmana stanica i primjenjenoj koncentraciji. Prooksidativan je učinak povezan s citotoksičnošću. Na genotoksičan učinak su najotpornije stanice Caco-2, a Cal 27 i Hep G2 pokazuju jasnu ovisnost genotoksičnog učinka THC-a o produljenju vremena inkubacije i povećanju koncentracije.

Abstract (english)

Colorectal cancer is the most common cause of death, after cardiovascular diseases which affects both men and women equally. Colorectal cancer treatment involves a surgical operation with use of chemotherapy and radiation. THC is the major active compound of Cannabis sativa plant which has been increasingly used as a support for chemotherapy. The aim of this study is to determine cytotoxic, pro-oxidative and genotoxic effects of THC. Human cell lines of oral epithelial carcinoma cells (Cal 27), hepatocellular liver carcinoma cells (Hep G2) and colon epithelial adenocarcinoma cells (Caco-2) were used as test systems. Cells were exposed to THC 2 – 48 hours, in concentration range from 0,33 μg mL-1 to 800 μg mL-1. It has been shown that cytotoxicity of THC depends on the duration of cell treatments and concentration used. Pro-oxidative effect was related with cytotoxicity. Caco-2 cells are most resistant to genotoxicity, and Cal 27 and Hep G2 cells show a clear connection of genotoxic effect of THC with the prolonging of incubation time and concentration used.

• Keywords: THC; citotoksičnost; indukcija slobodnih radikala; genotoksičnost; stanične linije; Cal 27; Hep G2; Caco-2; in vitro testovi
• Keywords (english): THC; cytotoxicity; induction of free radicals; genotoxicity; Cal 27; Hep G2; Caco-2; cell lines; in vitro assays
• Language: croatian
• URN:NBN: urn:nbn:hr:159:793152
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2019-12-11 13:07:23