In vitro istraživanje adjuvantnog učinka quisinostata na citotoksičnost kapecitabina na tumorske stanice mliječne žlijezde miša

master's thesis

• Title (english): In vitro study of the adjuvant effect of quisinostat on the cytotoxicity of capecitabine on mouse mammary gland tumor cells
• Author: Ivona Marjanović
• Mentor: Lidija Šver (mentor)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Biology and Microbial Genetics); Zagreb
• Defense date and country: 2019-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Zbog veće sličnosti uvjeta 3D staničnih kultura in vivo uvjetima, raste interes za njihovu primjenu u raznim područjima. Pokazano je da učinak antitumorskih lijekova u 2D staničnim kulturama nije isti učinku in vivo ili u 3D staničnim kulturama. Razlika učinkovitosti je vjerojatno posljedica fizičkih i fizioloških osobitosti tumorskih stanica, uslijed razlika u ekspresiji gena (koje su direktna posljedica djelovanja epigenskih mehanizama) ili zastupljenosti stanica u pojedinim fazama ţivotnog ciklusa. U ovom radu istraţen je utjecaj antitumorskog lijeka kapecitabina i pomoćne tvari quisinostata na stanice raka dojke miša u 2D i 3D staničnim kulturama. Rezultati su pokazali da kapecitabin ima o koncentraciji ovisan citotoksični učinak na stanice raka mliječne ţlijezde miša, izraţeniji u 2D kulturi. Dodatak quisinostata u obliku istovremenog tretmana s kapecitabinom ne utječe značajno na povećanje citotoksičnosti kapecitabina u 2D kulturi. MeĎutim, u 3D staničnoj kulturi dodatak quisinostata značajno je povećao citotoksičnost kapecitabina. Primjena quisinostata kao predtretmana, za razliku od 3D kulture, znatno je povećala citotoksičnost kapecitabina u 2D kulturi.

Abstract (english)

Due to the greater similarity of 3D cell culture model to in vivo conditions, there is increasing interest in it's application in various fields. The effect of antitumor drugs in 2D cell cultures has been shown to differ in comparison to in vivo or in 3D cell culture settings. The difference in efficacy is probably due to the physical and physiological characteristics of the tumor cells, due to differences in gene expression (which are a direct consequence of the action of epigenetic mechanisms) or the presence of cells at certain stages of the life cycle. The effect of the anticancer drug capecitabine and the adjuvant quisinostat on mouse breast cancer cells in 2D and 3D cell cultures was examined. The results showed that capecitabine had a concentration-dependent cytotoxic effect on mouse breast cancer cells, more expressed in 2D culture. The addition of quisinostat in the combination with capecitabine did not significantly increase the cytotoxicity in 2D culture. However, in 3D cell culture, the addition of quisinostat significantly increased the cytotoxicity of capecitabine. The use of quisinostat as pretreatment significantly increased the cytotoxicity of capecitabine in 2D culture only.

• Keywords: kapecitabin; quisinostat; inhibitor histon deacetilaza; 2D stanične kulture; 3D stanične kulture
• Keywords (english): capecitabine; quisinosat; histone deacetylase inhibitor; 2D cell cultures; 3D cell cultures
• Language: croatian
• URN:NBN: urn:nbn:hr:159:818387
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-13 12:10:13