Ispitivanje učinka kationiziranih i antimikrobno obrađenih tekstilija na vijabilnost i proliferaciju HaCaT stanične linije

master's thesis

• Title (english): The effects of cationized and antimicrobial processed textiles on the viability and proliferation of HaCaT cell line
• Author: Ivana Holetić
• Mentor: Teuta Murati (mentor)
• Committee member: Ivana Kmetič (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Chemistry and Biochemistry); (Laboratory for Toxicology); Zagreb
• Defense date and country: 2020-02-28, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Nutrition

Abstract

Zbog povećane bakterijske rezistencije se javila potreba za razvojem antimikrobno obrađenih tekstilija koje bi se koristile u bolnicama i zdravstvenim ustanovama. Međutim, antimikrobne tvari koje se koriste za obradu tekstilija se moraju pokazati sigurnima za ljudsku upotrebu. S obzirom da se radi o tekstilijama, prvi sustav koji dolazi u kontakt s antimikrobnim agensom je koža. Stoga se mora osigurati da neće biti toksičan za kožu te da neće izazivati alergije i iritacije. U ovom radu je ispitana potencijalna toksičnost tvari kojima su impregnirane tekstilije koristeći staničnu liniju humanih keratinocita HaCaT. MTT metoda je korištena u svrhu određivanja potencijalnog negativnog učinka tvari na proliferaciju i staničnu vijabilnost. Uočena je manja citotoksičnost kod tekstilija koje su oprane 10 puta u odnosu na one samo jednom isprane, pretpostavlja se zbog ispiranja potencijalno toksičnih tvari s tekstilije. Kako bi se odredio udio živih, mrtvih te rano i kasno apoptotičnih stanica korištena je metoda protočne citometrije.

Abstract (english)

Due to increased bacterial resistance, there was a need for the development of antimicrobial processed textiles for use in hospitals and healthcare facilities. However, antimicrobials used in textile processing must be safe for human use. Since the skin is first system that comes in contact with an antimicrobial agent impregnated in textile, it must be ensured that they will not cause dermal toxicity, allergies or irritations. In this work, the potential toxicity of textile impregnated substances using HaCaT cell line - human keratinocytes was examined. The MTT method was used to determine potential negative effect of s substance on proliferation and cell viability. It was observed that textiles that were washed 10 times were more toxic than the rinsed ones, presumably due to the leaching of potentially toxic substances from the textile. Flow cytometry was used to determine the percentage of living, dead, and early and late apoptotic cells.

• Keywords: HaCaT; kitozan; MTT metoda; protočna citometrija; antimikrobno obrađene tekstilije
• Keywords (english): HaCaT; chitosan; MTT method; flow cytometry; antimicrobial processed textiles
• Language: croatian
• URN:NBN: urn:nbn:hr:159:381248
• Study programme: Title: Nutrition; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-28 13:43:23