Uloga proteina uključenih u adheziju u otpornosti stanica raka prostate LNCaP na ionizirajuće zračenje

master's thesis

• Title (english): The role of adhesion proteins in the resistance of prostate carcinoma cell line LNCaP to ionizing radiation
• Author: Isabela Pehar
• Mentor: Andreja Ambriović Ristov (mentor); MBZ: 173311
• Committee member: Kristina Radošević (predsjednik povjerenstva)
• Granter: University of Zagreb; Faculty of Food Technology and Biotechnology; (Department of Biochemical Engineering); (Laboratory for Cell Technology, Application and Biotransformations); Zagreb
• Defense date and country: 2020-07-07, Croatia
• Scientific / art field, discipline and subdiscipline: BIOTECHNICAL SCIENCES; Biotechnology

Abstract

Karcinom prostate većinom se uspješno liječi radioterapijom koja postaje neučinkovita ukoliko stanice razviju otpornost. Jedan od mehanizama koji doprinosi nastanku otpornosti stanica na radioterapiju je promjena u adheziji stanica posredovana integinima. Kompleks adhezije integrina je multimolekularni kompleks koji se zajednički naziva adhesom i povezuje se s razvojem zloćudnih tumora, ali i s promjenom osjetljivost stanica na zračenje. Stoga se ciljanje adhesoma može iskoristiti za povećanje osjetljivosti tumorskih stanica na radioterapiju. Cilj ovog rada bio je usporediti sastav adhesoma stanične linije karcinoma prostate LNCaP i sublinije otporne na radioterapiju LNCaP RR. Izolirani su kompleksi adhezije integrina obje stanične linije nakon kemijskog umrežavanja i uspoređeni Western blot analizom. U stanicama LNCaP RR otpornim na radioterapiju primijećena je povećana ekspresija paksilina. Ovim radom stečeni su preduvjeti za buduću analizu kompleksa adhezije integrina ovog staničnog modela spektrometrijom masa koji bi mogli doprinijeti razumijevanju uloga proteina adhesoma u razvoju otpornosti stanica na radioterapiju.

Abstract (english)

Prostate cancer is mostly successfully treated with radiotherapy which becomes ineffective if the cells develop resistance. One of the mechanisms that contributes to the development of cell resistance to radiotherapy is a change in cell adhesion mediated by integin. The integrin adhesion complex is a multimolecular complex called adhesome, and is associated with the development of malignant tumors, but also with a change in the sensitivity of cells to radiation. Therefore, targeting the adhesome can be used to increase the sensitivity of tumor cells to radiotherapy. The aim of this study was to compare the composition of the LNCaP prostate cancer cell line adhesome and the LNCaP RR radiotherapy-resistant subline. The integrin adhesion complexes of both cell lines were isolated after chemical crosslinking and compared by Western blot analysis. An increased expression of paxilin was observed in LNCaP RR cells. This study provides the preconditions for future analysis of the integrin adhesion complex of this cell model by mass spectrometry, which could contribute to the understanding of the role of adhesome proteins in the development of cell resistance to radiotherapy.

• Keywords: karcinom prostate; adhezija stanica; integrini; adhesom; radiorezistencija
• Keywords (english): prostate cancer; cell adhesion; integrins; adhesome; radioresistance
• Language: croatian
• URN:NBN: urn:nbn:hr:159:052714
• Study programme: Title: Molecular Biotechnology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-07-08 10:26:48